1. 重组蛋白
  2. Complement System
  3. Complement Component 3
  4. Complement Component 3a
  5. Complement C3/C3a 蛋白, Human

Complement C3/C3a 蛋白, Human

目录号: HY-P7862
COA 产品使用指南

补体 C3/C3a 蛋白通过 C3 转化酶在经典途径和替代途径中的加工,在启动补体系统中发挥关键作用。激活后,C3b 与表面共价结合,而 C3a(C3 蛋白水解产生的过敏毒素)介导局部炎症。Complement C3/C3a 蛋白, Human 是重组的 补体 C3/C3a 蛋白,由 E. coli 表达,不带标签。Complement C3/C3a 蛋白, Human 全长 77 个氨基酸,分子量约为 ~13.0 kDa。

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规格 价格 是否有货 数量
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5 μg ¥1040 In-stock
10 μg ¥1760 In-stock
50 μg ¥4950 In-stock
100 μg ¥8400 In-stock
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描述

Complement C3/C3a proteins play a key role in initiating the complement system through processing by C3 convertase in the classical and alternative pathways. Upon activation, C3b covalently binds to the surface, while C3a (anaphylatoxin produced by proteolysis of C3) mediates local inflammation. Complement C3/C3a Protein, Human is the recombinant human-derived Complement C3/C3a protein, expressed by E. coli , with tag free. The total length of Complement C3/C3a Protein, Human is 77 a.a., with molecular weight of ~13.0 kDa.

研究背景

C3 在补体系统的启动中发挥着关键作用,C3 转化酶对其进行的加工构成了经典补体途径和替代补体途径中的中心反应。激活后,C3b 能够通过其反应性硫酯共价结合细胞表面碳水化合物或免疫聚集体。同时,源自补体 C3 蛋白水解降解的过敏毒素 C3a 充当局部炎症过程的介质。在慢性炎症的情况下,C3a 充当中性粒细胞的化学引诱剂,诱导平滑肌收缩,增加血管通透性,并触发肥大细胞和嗜碱性白细胞释放组胺。

生物活性

Measured by its ability to enhance IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is ≤0.4476 μg/mL, corresponding to a specific activity is ≥2234.1376 U/mg.

  • Measured by its ability to enhance IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 0.1759 μg/mL, corresponding to a specific activity is 5685.0483 U/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P01024 (S672-R748)

基因 ID

718  [NCBI]

蛋白结构
N-term
C3a (S672-R748)
Accession # P01024
C-term
同用名
rHuComplement C3/C3a; Complement Component C3a; C3a; Anaphylatoxin
氨基酸序列

SVQLTEKRMDKVGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHARASHLGLAR

分子量

Approximately 13.0 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Complement C3/C3a Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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