CTXB 蛋白, Vibrio cholerae

The pentameric ring of the B subunit of the CTXB protein guides the A subunit by binding to GM1 ganglioside on intestinal epithelial cells. This loop binds five GM1 gangliosides and promotes the specific interaction of the toxin with its cellular receptor. CTXB Protein, Vibrio cholerae is the recombinant CTXB protein, expressed by E. coli , with tag free. The total length of CTXB Protein, Vibrio cholerae is 103 a.a., with molecular weight of ~11.0 kDa.

CTXB 蛋白以其 B 亚基五聚环为代表，通过与肠上皮细胞表面的 GM1 神经节苷脂结合，在引导 A 亚基到达其靶点方面发挥着关键作用。五聚环善于结合五种 GM1 神经节苷脂，促进毒素与其细胞受体之间的特异性相互作用。值得注意的是，CTXB B 亚基本身缺乏毒性活性。全毒素被称为胆原，包含 B 亚基的五聚环，形成中心孔被 A 亚基占据的结构。A 亚基本身由两条链 A1 和 A2 组成，通过二硫桥连接，进一步强调了这种毒素的复杂结构和功能。

Others

E. coli

Tag Free

P01556 (T22-N124)

57740119

TPQNITDLCAEYHNTQIYTLNDKIFSYTESLAGKREMAIITFKNGAIFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMAN

Approximately 11.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, pH8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.