1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Cutinase 蛋白, Thermobifida Fusca (His)

Cutinase 蛋白, Thermobifida Fusca (His)

目录号: HY-P7829
COA 产品使用指南

角质酶蛋白催化角质(一种植物角质层聚酯)的水解。Cutinase 蛋白, Thermobifida Fusca (His) 是重组的 角质酶蛋白,由 E. coli 表达,带有 C-6*His 标签。Cutinase 蛋白, Thermobifida Fusca (His) 全长 261 个氨基酸,分子量为 28-30 kDa。

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规格 价格 是否有货 数量
10 μg ¥1654 In-stock
50 μg ¥4962 询价
100 μg   询价  

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描述

Cutinase protein catalyzes the hydrolysis of cutin, a plant cuticle polyester. Cutinase Protein, Thermobifida Fusca (His) is the recombinant Cutinase protein, expressed by E. coli , with C-6*His labeled tag. The total length of Cutinase Protein, Thermobifida Fusca (His) is 261 a.a., with molecular weight of 28-30 kDa.

研究背景

角质酶蛋白在细胞过程中发挥着关键作用,催化角质的水解,角质是构成植物角质层结构的关键聚酯。正如各种研究中记载的那样,角质酶对对硝基苯酚连接的脂肪族酯(pNP-脂肪族酯)表现出酯酶活性,显示出对短链底物的偏好。此外,角质酶通过水解甘油三酯(如三醋精、三丁酸甘油酯、三癸酸甘油酯和三月桂酸甘油酯)而展现出多功能性,并且对短链底物具有显着的亲和力。此外,该酶具有水解半纤维素木聚糖的能力,并且能够熟练降解广泛使用的塑料聚对苯二甲酸乙二醇酯 (PET)。此外,角质酶可以解聚聚(ε-己内酯)(PCL),这是一种合成脂肪族可生物降解聚酯,突出了其在各种生物降解过程中的作用。角质酶广泛的底物特异性强调了其在涉及天然聚合物分解和合成聚合物降解的酶反应中的重要性,展示了其在环境和工业环境中的潜在应用。

生物活性

Measured by its ability to catalyze a substrate, p-nitrophenol succinate, which can be measured by absorbance at 410 nm. The specific activity is 42.67 U/mL, as measured under the described conditions.

种属

Others

表达系统

E. coli

标签

C-6*His

蛋白编号

E5BBQ3 (A1-F261)

基因 ID

/

蛋白结构
N-term
Cutinase (A1-F261)
Accession # E5BBQ3
6*His
C-term
同用名
rThCutinase, His; Cutinase
氨基酸序列

ANPYERGPNPTDALLEARSGPFSVSEENVSRLSASGFGGGTIYYPRENNTYGAVAISPGYTGTEASIAWLGERIASHGFVVITIDTITTLDQPDSRAEQLNAALNHMINRASSTVRSRIDSSRLAVMGHSMGGGGSLRLASQRPDLKAAIPLTPWHLNKNWSSVTVPTLIIGADLDTIAPVATHAKPFYNSLPSSISKAYLELDGATHFAPNIPNKIIGKYSVAWLKRFVDNDTRYTQFLCPGPRDGLFGEVEEYRSTCPF

分子量

28-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Cutinase Protein, Thermobifida Fusca (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cutinase Protein, Thermobifida Fusca (His)
目录号:
HY-P7829
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