DKK1 蛋白, Human (HEK293, His-Avi)

DKK-1 Proteinas, a potent antagonist of canonical Wnt signaling, inhibits LRP5/6-Wnt interaction and forms a ternary complex with KREMEN, facilitating LRP5/6 internalization. Beyond antagonizing KREMEN1's pro-apoptotic function independently of Wnt, DKK-1 exhibits anti-apoptotic activity. Implicated in limb development, it modulates Wnt signaling for normal limb patterning. Interacting with LRP5/6 through its C-terminal Cys-rich domain, DKK-1 attenuates Wnt-mediated signaling influenced by MESD and/or KREMEN, emphasizing its multifaceted role in regulating cellular processes. DKK1 Protein, Human (HEK293, His-Avi) is the recombinant human-derived DKK1 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of DKK1 Protein, Human (HEK293, His-Avi) is 235 a.a., with molecular weight of 45-55 kDa.

DKK1 蛋白通过多种机制发挥经典 Wnt 信号传导的有效拮抗剂的作用。它抑制LRP5/6和Wnt之间的相互作用，并与跨膜蛋白KREMEN形成三元复合物，促进LRP5/6的内化。值得注意的是，DKK1 不仅以不依赖 Wnt 的方式拮抗 KREMEN1 的促凋亡功能，而且还表现出抗凋亡活性。该蛋白参与肢体发育，调节 Wnt 信号以确保正常的肢体模式。通过其 C 端富含 Cys 的结构域，DKK1 与 LRP5 和 LRP6 相互作用，特别是与 LRP5 的 β 螺旋桨区域 3 和 4 结合。这种相互作用进一步受到 MESD 和/或 KREMEN 的影响，共同导致 Wnt 介导的信号传导减弱。此外，DKK1 与 LRP6 和 KREM1 形成三元复合物，突显其在调节关键细胞过程以及与 Wnt 信号转导中涉及的关键蛋白相互作用方面的多方面作用。

Human

HEK293

C-Avi;C-His

O94907 (T32-H266)

22943  [NCBI]

45-55 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.