1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Bone Morphogenetic Proteins (BMPs)
  5. BMP-11/GDF-11
  6. GDF-11/BMP-11 蛋白, Human (HEK293)

GDF-11/BMP-11 蛋白, Human (HEK293)

目录号: HY-P70222
COA 产品使用指南

骨形态发生蛋白 11 (BMP-11; GDF11),又称生长/分化因子 11,是一种多效配体蛋白,属于 TGFβ 家族。GDF-11/BMP-11 信号通过激活素受体 I 型、II 型,导致 SMAD2SMAD3 磷酸化,激发信号。GDF-11/BMP-11 是中枢神经系统 (CNS) 形成和命运的重要调节因子。在老年大脑中,外源性外周传递的 GDF-11/BMP-11 可能增强神经发生和血管生成,并改善神经病理结果。人 GDF-11/BMP-11 蛋白全长 407 个氨基酸 (M1-S407),具有 1 个糖基化片段。重组人 GDF-11/BMP-11 蛋白 (HEK293) 全长 109 个氨基酸 (N299-S407),由 HEK293细胞表达。

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50 μg ¥4300 询价
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描述

Bone morphogenetic protein 11 (BMP-11; GDF11), also known as growth/differentiation factor 11, is a polymorphic ligand protein belonging to the TGFβ family. The GDF-11/BMP-11 signal activates the signal through activator receptor types I and II, resulting in the phosphorylation of SMAD2 and SMAD3[1]. GDF-11/BMP-11 is an important regulator of central nervous system (CNS) formation and fate[2]. Exogenous peripheral delivery of GDF-11/BMP-11 may enhance neurogenesis and angiogenesis and improve neuropathological outcomes in the elderly brain[2]. The total length of human GDF-11/BMP-11 protein is 407 amino acids (M1-M407), with a glycosylation domain. GDF-11/BMP-11 Protein, Human (HEK293) has a total length of 109 amino acids (N299-S407), is expressed in HEK293 cells.

研究背景

骨形态发生蛋白11(BMP-11;GDF11)又称生长/分化因子11,是一种多效性配体蛋白,属于TGFβ家族。BMP-11 通过 II 型激活素受体、ACVR2A 和 ACVR2B 以及 I 型激活素受体、ACVR1B、ACVR1C 和 TGFBR1 发出信号,导致 SMAD2SMAD3 磷酸化[1]
BMP- 11 与生长/分化因子 8 (GDF8) 高度相似,并且比 GDF8 表现出更有效的 SMAD2/3 激活剂,并且通过 I 型激活素样受体激酶受体 ALK4/5/7 更有效地发出信号。此外,GDF-11/BMP-11 的信号转导受细胞外蛋白拮抗剂控制,包括 FS、FSTL3、GASP1 和 GASP2[1]
GDF-11/BMP-11 发挥作用在发育过程中发挥关键作用,包括前/后模式、肾、胃、脾和内分泌胰腺的形成。在胚胎中,BMP-11也在腭上皮中表现出强表达,包括腭架的内侧边缘上皮和中线上皮缝。在整个腭架和舌间充质中也可见到不太明显的表达[3]
最近发现 GDF-11/BMP-11 在成人中枢神经系统 (CNS) 中表达[3],是中枢神经系统形成和命运的重要调节因子[2]。在衰老的大脑中,外源性、外周递送的 GDF-11/BMP-11 可能会增强神经发生和血管生成,并改善神经病理结果。外源性增加循环 GDF-11/BMP-11 浓度可能是改善大脑衰老和神经病理学有害方面的可行方法[2]

体外研究
(In Vitro)

"GDF-11/BMP-11 (5, 10, 20, and 40 ng/mL; 7 d) maintains the colony and cellular morphology of undifferentiated human embryonic stem cells (hESC), maintains POU5f1, NANOG, TRA-1-60, and SSEA4 expression, and displays increased SMAD2/3 phosphorylation in serum-free medium at 20 ng/mL concentration[4].
GDF-11/BMP-11 (50-400 ng/mL; 2 d) induces browning of 3T3-L1 adipocytes via coordination of multiple signalling pathways, including mTORC1-COX2 and p38MAPK-PGC-1α as non-canonical pathways, as well as Smad1/5/8 as a canonical pathway[5].
"

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

O95390 (N299-S407)

基因 ID
蛋白结构
N-term
BMP-11 (N299-S407)
Accession # O95390
C-term
中文名
重组人 GDF-11/BMP-11 蛋白 (HEK293)
同用名
rHuGrowth/differentiation factor 11; Growth/differentiation factor 11; GDF-11; Bone morphogenetic protein 11; BMP-11
氨基酸序列

NLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS

分子量

Approximately 14.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 50% glycerol, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GDF-11/BMP-11 Protein, Human (HEK293)
目录号:
HY-P70222
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