1. 重组蛋白
  2. Fc Receptors Biotinylated Proteins
  3. Immunoglobulin Fc Region
  4. IgG1
  5. IgG1 蛋白, Human (Biotinylated, C103S, HEK293)

IgG1 蛋白, Human (Biotinylated, C103S, HEK293)

目录号: HY-P77526
产品使用指南

IgG1蛋白是免疫球蛋白重链的恒定区,在体液免疫识别阶段充当受体。它触发 B 淋巴细胞克隆扩增并分化为分泌免疫球蛋白的浆细胞。IgG1 蛋白, Human (Biotinylated, C103S, HEK293) 是重组的 IgG1 蛋白,由 HEK293 表达,不带标签。IgG1 蛋白, Human (Biotinylated, C103S, HEK293) 全长 232 个氨基酸,分子量约为 ~26.1 KDa。

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重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

The IgG1 protein is the constant region of the immunoglobulin heavy chain and serves as a receptor during the recognition phase of humoral immunity. It triggers clonal expansion and differentiation of B lymphocytes into immunoglobulin-secreting plasma cells. IgG1 Protein, Human (Biotinylated, C103S, HEK293) is the recombinant human-derived IgG1 protein, expressed by HEK293 , with tag free. The total length of IgG1 Protein, Human (Biotinylated, C103S, HEK293) is 232 a.a., with molecular weight of ~26.1 KDa.

研究背景

免疫球蛋白重链的恒定区(称为抗体)代表 B 淋巴细胞产生的膜结合或分泌糖蛋白。在体液免疫的识别阶段,这些膜结合的免疫球蛋白充当受体,在结合特定抗原后启动B淋巴细胞的克隆扩增和分化为分泌免疫球蛋白的浆细胞。分泌的免疫球蛋白在体液免疫的效应阶段发挥着至关重要的作用,导致结合抗原的消除。抗原结合位点由一条重链的可变结构域及其相关轻链的可变结构域形成,导致每种免疫球蛋白具有两个对特定抗原具有显着亲和力的抗原结合位点。可变结构域经历 V-(D)-J 重排和随后的体细胞超突变,从而在暴露和选择后实现特定抗原的亲和力成熟。IgG1 蛋白介导单核细胞的效应功能,触发病毒感染细胞的抗体依赖性细胞毒性 (ADCC)。免疫球蛋白由两条相同的重链和两条相同的轻链组成,通过二硫键互连,并与 FCGR1A、FCGR2A 和 FCGR3A 相互作用以介导各种效应功能。

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P01857-1 (E99-K330, C103S)

基因 ID
蛋白结构
N-term
IgG1 (E99-K330)
Accession # P01857
C-term
同用名
Immunoglobulin heavy constant gamma 1; IGHG1
分子量

Approximately 26.1 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IgG1 Protein, Human (Biotinylated, C103S, HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IgG1 Protein, Human (Biotinylated, C103S, HEK293)
目录号:
HY-P77526
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