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  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-17
  5. IL-17A IL-17F
  6. IL-17A (155a.a)-17F Heterodimer 蛋白, Human (HEK293, His)

IL-17A (155a.a)-17F Heterodimer 蛋白, Human (HEK293, His)

目录号: HY-P73178
产品使用指南

IL-17A-17F 异二聚体蛋白是细胞因子 IL-17A 和 IL-17F 的异二聚体。 IL-17A 和IL-17F 都能诱导抗菌肽、细胞因子(IL-6 和 GM-CSF)、趋化因子 (CCL2、CCL7 和CXCL1) 和基质金属蛋白酶 (MMP-1 和 MMP13)。IL-17A-17F 的生物学活性介于 IL-17A 和IL-17F 之间。IL-17A (155a.a)-17F Heterodimer 蛋白, Human (HEK293, His) 是一种重组人 IL-17A-17F 异二聚体蛋白,C 端带有 His 标签,在HEK293 细胞中表达,由 288 个氨基酸组成 (IL-17A (M1-A155) & IL-17F (R31-Q163))。

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描述

IL-17A-17F Heterodimer Protein is the heterodimer of the cytokines IL-17A and IL-17F. Both IL-17A and IL-17F can induce antimicrobial peptides, cytokines (IL-6 and GM-CSF), chemokines (CCL2, CCL7 and CXCL1), and matrix metalloproteinases (MMP-1 and MMP13). IL-17A-17F shows intermediate biological activity between IL-17A and IL-17F[1][2][3][4]. IL-17A (155a.a)-17F Heterodimer Protein, Human (HEK293, His) is a recombinant human IL-17A-17F heterodimer protein with His tag at the C-terminus and is expressed in HEK293 cells. It consists of 288 amino acids (IL-17A (M1-A155) & IL-17F (R31-Q163)).

研究背景

IL-17A-17F Heterodimer Protein is the heterodimer of the cytokines IL-17A and IL-17F. Both IL-17A and IL-17F belongs to the IL-17 cytokine family. IL-17A-17F heterodimer, IL-17A and IL-17F homodimers can be produced by differentiated Th17 cells[1][2]. IL-17F shares the most similarities with IL-17A (50% homology)[2]. Both IL-17A and IL-17F can induces antimicrobial peptides, cytokines (IL-6 and GM-CSF), chemokines (CCL2, CCL7 and CXCL1), and matrix metalloproteinases (MMP-1 and MMP13)[2][3]. IL-17A, IL-17F and IL-17A-17F use the same receptor complex: IL-17RA and IL-17RC heterodimer. And they trigger qualitatively similar signaling pathways. IL-17A-17F shows intermediate biological activity between IL-17A (most potent) and IL-17F (least potent)[2][4].

体外研究
(In Vitro)

IL-17F/IL-17A (5 ng/mL; 16-24 h) induces GRO-, IL-6, and IL-8 secretion in human primary foreskin fibroblast (BJ) cells[5].
IL-17F/IL-17A binds to IL-17RA.Fc with an EC50 of 329 ng/mL, IL-17A and IL-17F bind to IL-17RA.Fc with EC50s of 19 ng/mL and > 2000 ng/mL, respectively[5].

生物活性

1.Measured by its binding ability in a functional ELISA. 2.Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα and the ED50 is 0.8-4 ng/mL.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q16552 (M1-A155)&Q96PD4 (R31-Q163)

基因 ID

3605  [NCBI]&112744  [NCBI]

中文名
重组人白介素17A-17F 异二聚体蛋白 (HEK293, His)
同用名
CTLA-8; IL-17; Interleukin-17A; IL17A; Interleukin-17F; IL-17F; Cytokine ML-1
分子量

Approximately 31 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-17A (155a.a)-17F Heterodimer Protein, Human (HEK293, His) 相关分类

  • 复溶计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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