1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. IL-1R IL-1R1/CD121a
  5. Type I IL-1 Receptor (IL-1R1)
  6. IL-1R1 蛋白, Human (332a.a, HEK293, His)

IL-1R1 蛋白, Human (332a.a, HEK293, His)

目录号: HY-P73186
产品使用指南

IL-1R1 (CD121a) 是一种 IL-1 受体,与 IL-1α, IL-1β, IL-1Ra, 和 IL-38 结合的亲和力相当 (Kd: 0.1-1 nM)。IL-1R1 可调节多种促炎细胞因子的转录并介导免疫和炎症反应,包括 NF-κB 和 MAPK 信号通路的激活。IL-1R1 蛋白, Human (332a.a, HEK293, His) 是一种由 HEK293 细胞产生的重组人 IL-1R1 的胞外片段 (M1-T332),其 C 末端带有 His 标签。

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描述

IL-1R1 (CD121a) belongs to IL-1 receptor, and binds to IL-1α, IL-1β, IL-1Ra and IL-38 with comparable affinities (Kd: 0.1-1 nM)[1]. IL-1R1 regulates the transcription of multiple proinflammatory cytokines and mediates immune and inflammatory responses, including the activation of NF-κB and MAPK pathway[4]. IL-1R1 Protein, Human (332a.a, HEK293, His) is a recombinant human extracellular region of IL-1R1 (M1-T332) with a C-Terminal His tag, which is produced in HEK293 cells.

研究背景

IL-1R1 (CD121a), a member of IL-1 receptor, is a receptor for IL-1α, IL-1β, IL-1Ra and IL-38, thereby mediating IL-1-dependent activation[1]. IL-1R1 is expressed in specific endothelial cells, ventricular cells, astrocytes, and neurons[2].
The sequence of amino acids in IL-1R1 differs in different species. Human IL-1R1 shares <70% aa sequence identity with mouse and rats.
IL-1R1 binds to IL-1 by forming a heterodimer with IL-1R3 (IL-1RAcP). The heterodimer is responsible for the signal transduction by the hydrolysis of GTP, followed by JNK and p38 MAP kinase[3]. Upon binding of IL-1, IL-1R1 initiates the downstream signaling cascade (MAPK p38 and NF-κB pathway)[4]. IL-1R1 signaling induces human Th17 cell differentiation and commitment, which is crucial in the development of autoimmune diseases[4].
IL-1R1 mediates immune and inflammatory responses. IL-1R1 mediates the activation of NF-κB, MAPK pathway.

生物活性

Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b and the EC50 is typically 40-200 ng/mL.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P14778 (M1-T332)

基因 ID

3554  [NCBI]

中文名
重组人 (332a.a, HEK293, His) 白介素-1 受体 1
同用名
Interleukin-1 receptor type 1; IL-1R-1; CD121a; IL1R1; IL1R; IL1RA; IL1RT1
分子量

51-57 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-1R1 Protein, Human (332a.a, HEK293, His)
目录号:
HY-P73186
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