1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors NK Cell CD Proteins
  4. IL-2 Receptor CD132/IL-2R gamma
  5. IL-2R gamma/Common gamma-Chain
  6. IL-2R gamma/CD132 蛋白, Human (sf9, His)

IL-2R gamma/CD132 蛋白, Human (sf9, His)

目录号: HY-P73204
产品使用指南

IL-2R gamma (CD132),一种表达于白细胞亚群的 Ⅰ 型细胞因子受体,是 IL-2 的受体。IL-2R gamma 可与 IL-2R beta 形成异二聚体,并增加 IL-2R beta 对 IL-2 的亲和力。IL-2R gamma 在淋巴细胞及其他类型细胞的发育、活化、增殖、分化和调控中发挥着重要作用。IL-2R gamma/CD132 蛋白, Human (sf9, His) 是一种由 Sf9 昆虫细胞产生的重组人 IL-2R gamma 的胞外片段 (M1-N254),其 C 末端带有 His 标签。

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描述

IL-2R gamma (CD132), a type I cytokine receptor expressed on leucocyte subsets, is a receptor for IL-2. IL-2R gamma forms heterodimer with IL-2R beta, and increases the affinity of IL-2R beta for IL-2[1]. IL-2R gamma plays an important role in the development, activation, proliferation, differentiation and regulation of lymphocytes and other cell types[2]. IL-2R gamma/CD132 Protein, Human (sf9, His) is a recombinant human extracellular region of IL-2R gamma (M1-N254) with a C-Terminal His tag, which is produced in Sf9 insect cells.

研究背景

IL-2R gamma (CD132), a receptor for IL-2, is a member of the type I cytokine receptor family and type 5 subfamily. IL-2R gamma is expressed in leucocyte subsets and human monocytes[1][3]. Human IL-2R gamma consists of extracellular domain (L23-A262), helical domain (V263-L283), and cytoplasmic domain (E284-T369).
The sequence of amino acids in IL-2R beta differs in different species. Human IL-2R gamma shares <75% aa sequence identity with mouse and rats.
IL-2R gamma has low-affinity for IL-2, but has intermediate affinity for IL-2 when forming heterodimer with IL-2R beta. IL-2R beta/gama heterodimer complex transduces a signal when IL-2 concentrations are relatively high[1]. IL-2R gamma can be utilized by the IL-2, IL-4, IL-7, IL-9, and IL-15 receptor, and takes part in the development, activation, proliferation, differentiation and regulation of lymphocytes and other cell types[2]. IL-2R gamma is tightly up-regulated by IL-2 and IFN gamma[3]. Mutations of L-2R gamm cause human X-linked severe combined immunodeficiency (XSCID)[4].
IL-2R gamma is involved in inflammatory response, and mediates activation of the cells[1].

体外研究
(In Vitro)

IL-2R gamma (human) migrates with a molecular weight corresponding to 55–60 kDa (immunoblotting of lysates prepared from Sf9 cells infected with VL1392-hIL-2Rγ)[5].
IL-2R gamma binds to IL-2 in recombinant IL-2R gamma plasmid (pGEX-4T-1-IL-2Rγ)-transfected 293T cells[6].

生物活性

Measured by its ability to bind rat IL2 in a functional ELISA.

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P31785 (M1-N254)

基因 ID

3561  [NCBI]

中文名
重组人 (sf9, His) 白介素-2 受体 gamma/CD132
同用名
IL-2RG; IL-2 R gamma; IL-2Rγ; Cytokine receptor common subunit gamma; CD132; IL2RG
分子量

Approximately 43 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-2R gamma/CD132 Protein, Human (sf9, His)
目录号:
HY-P73204
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