Jagged-1/JAG1 蛋白, Human (HEK293, His)

Jagged-1/JAG1 protein is a ligand for multiple Notch receptors and crucially mediates Notch signaling through interactions with NOTCH1, NOTCH2, and NOTCH3. Its role in hematopoiesis suggests its involvement in the regulation of blood cell development. Jagged-1/JAG1 Protein, Human (HEK293, His) is the recombinant human-derived Jagged-1/JAG1 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Jagged-1/JAG1 Protein, Human (HEK293, His) is 1013 a.a., with molecular weight of 150 kDa.

Jagged-1 (JAG1) 蛋白作为多个 Notch 受体的配体，在介导 Notch 信号传导中发挥着至关重要的作用，其与 NOTCH1、NOTCH2 和 NOTCH3 的相互作用就证明了这一点。它参与造血过程中的细胞命运决定，表明它在血细胞发育中具有调节作用。JAG1 参与哺乳动物心血管发育的各个阶段，并且与该过程的早期和晚期阶段相关。此外，它还能抑制成肌细胞分化并增强体外成纤维细胞生长因子诱导的血管生成。值得注意的是，JAG1 和 NOTCH1 之间的相互作用是钙离子依赖性的，凸显了其在细胞过程中调节机制的复杂性。

Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells and the ED₅₀ is 0.3007-3.8 μg/mL.

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  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.3007 μg/mL in the presence of 500 ng/mL BMP-2, corresponding to a specific activity is 3.32×10³ U/mg

Human

HEK293

C-10*His

P78504/NP_000205.1 (Q34-S1046)

182  [NCBI]

QFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNS

150 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. ) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.