1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Kallikrein-8 蛋白, Human (232a.a, HEK293, His)

Kallikrein-8 蛋白, Human (232a.a, HEK293, His)

目录号: HY-P70169
产品使用指南

Kallikrein-8 是一种丝氨酸蛋白酶,可降解酪蛋白和纤维蛋白原等蛋白质。它会裂解 L1CAM,诱导神经突生长和肌束颤动。Kallikrein-8 蛋白, Human (232a.a, HEK293, His) 是重组的 激肽释放酶 8 蛋白,由 HEK293 表达,带有 C-6*His 标签。Kallikrein-8 蛋白, Human (232a.a, HEK293, His) 全长 232 个氨基酸,分子量约为 ~35.0 kDa。

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描述

Kallikrein-8 is a serine protease that degrades proteins such as casein and fibrinogen. It cleaves L1CAM, inducing neurite outgrowth and fasciculations. Kallikrein-8 Protein, Human (232a.a, HEK293, His) is the recombinant human-derived Kallikrein-8 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kallikrein-8 Protein, Human (232a.a, HEK293, His) is 232 a.a., with molecular weight of ~35.0 kDa.

研究背景

Kallikrein-8 是一种丝氨酸蛋白酶,具有降解多种蛋白质的能力,包括酪蛋白、纤维蛋白原、激肽原、纤连蛋白和 IV 型胶原蛋白。此外,它会响应神经活动的增强而裂解 L1CAM,从而诱导培养的海马神经元中的神经突生长和束颤。这种蛋白酶在谢弗侧支通路中孤儿和小突触纽带的形成和成熟中起着关键作用,调节海马体中谢弗侧支的长期增强,并且对于记忆获取和突触可塑性至关重要。此外,Kallikrein-8 还参与皮肤脱屑和角质形成细胞增殖,从而促进这些过程。此外,它在脊髓损伤后发病机制的第二阶段发挥着重要作用,强调了其在神经和皮肤过程中的多种功能。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAH40887.1 (Q29-G260)

基因 ID
蛋白结构
N-term
Kallikrein-8 (Q29-G260)
Accession # AAH40887.1
6*His
C-term
同用名
rHuKallikrein 8/KLK8, His; Kallikrein-8; hK8; Neuropsin; NP; Ovasin; Serine Protease 19; Serine Protease TADG-14; Tumor-Associated Differentially Expressed Gene 14 Protein; KLK8; NRPN; PRSS19; TADG14
氨基酸序列

QEDKVLGGHECQPHSQPWQAALFQGQQLLCGGVLVGGNWVLTAAHCKKPKYTVRLGDHSLQNKDGPEQEIPVVQSIPHPCYNSSDVEDHNHDLMLLQLRDQASLGSKVKPISLADHCTQPGQKCTVSGWGTVTSPRENFPDTLNCAEVKIFPQKKCEDAYPGQITDVMVCAGSSKGADTCQGDSGGPLVCDGALQGITSWGSDPCGRSDKPGVYTNICRYLDWIKKIIGSKG

分子量

Approximately 35.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Kallikrein-8 Protein, Human (232a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kallikrein-8 Protein, Human (232a.a, HEK293, His)
目录号:
HY-P70169
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