2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. LDHA 蛋白, Mouse (His)

LDHA 蛋白, Mouse (His)

目录号: HY-P73270
产品使用指南

LDHA 蛋白催化丙酮酸和乳酸同时且立体特异性的相互转化，同时伴随着 NADH 和 NAD(+) 的相互转化。LDHA 蛋白, Mouse (His) 是重组的 LDHA 蛋白，由 E. coli 表达，带有 N-His 标签。LDHA 蛋白, Mouse (His) 全长 332 个氨基酸，分子量约为 ~36.5 kDa。

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1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
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100 μg   询价  

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描述

LDHA protein catalyzes the simultaneous and stereospecific interconversion of pyruvate and lactate, accompanied by the reciprocal interconversion of NADH and NAD(+). LDHA Protein, Mouse (His) is the recombinant mouse-derived LDHA protein, expressed by E. coli , with N-His labeled tag. The total length of LDHA Protein, Mouse (His) is 332 a.a., with molecular weight of ~36.5 kDa.
研究背景

LDHA 蛋白是乳酸脱氢酶的一种亚型，在细胞代谢中发挥着关键作用。它催化丙酮酸转化为乳酸，在厌氧条件下产生能量。了解 LDHA 蛋白的功能对于研究代谢紊乱和制定针对癌症和其他疾病代谢改变的治疗策略至关重要。
种属

Mouse
表达系统

E. coli
标签

N-His
蛋白编号

P06151 (M1-F332)
基因 ID

16828  [NCBI]
蛋白结构
N-term
His
LDHA (M1-F332)
Accession # P06151
C-term
同用名
L-lactate dehydrogenase A chain; LDH-A; LDH-M; Ldha; Ldh-1
分子量

Approximately 36.5 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

LDHA Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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