N-Cadherin 蛋白, Human (699a.a, HEK293, His)

N-Cadherin Protein forms dimers with CDH2 chains, promoting cell-cell adhesion and sorting of cell types. It regulates neural stem cell quiescence by anchoring them to ependymocytes, disrupted by MMP24 cleavage. It also promotes cell-to-cell junctions and neurite branching, and regulates dendritic spine density. N-Cadherin Protein interacts with CDCP1, PCDH8, OBSCN, FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43, CTTN, and FBXO45. N-Cadherin Protein, Human (699a.a, HEK293, His) is the recombinant human-derived N-Cadherin protein, expressed by HEK293 , with C-His labeled tag. The total length of N-Cadherin Protein, Human (699a.a, HEK293, His) is 699 a.a., with molecular weight of 75-90 kDa.

N-钙粘蛋白是一种钙依赖性细胞粘附蛋白，通过与邻近细胞的 CDH2 链形成二聚体，在同型细胞间粘附中发挥至关重要的作用。钙粘蛋白之间的这种相互作用有助于不同细胞类型的分类。在成人室管膜下区，N-钙粘蛋白通过将神经干细胞锚定到室管膜细胞来调节神经干细胞的静止状态。然而，当被 MMP24 切割时，这种锚定被破坏，导致神经干细胞静止的调节。N-钙粘蛋白还在胰腺β细胞和神经嵴干细胞之间细胞间连接的形成中发挥作用，促进后者过程的延伸。此外，它对于适当的神经突分支以及突触前和突触后组织至关重要。N-钙粘蛋白可能参与神经元识别机制和海马神经元树突棘密度的调节。它可以与其他钙粘蛋白形成同二聚体或异二聚体，并且这些相互作用以反式发生，即与来自邻近细胞的钙粘蛋白链发生相互作用。N-钙粘蛋白与多种蛋白质相互作用，包括CDCP1、PCDH8和OBSCN，并且在包含FGFR4、NCAM1、CDH2、PLCG1、FRS2、SRC、SHC1、GAP43和CTTN的复合物中被发现。此外，它已被证明可以与 FBXO45 交互。

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.

Human

HEK293

C-His

P19022 (S26-A724)

1000  [NCBI]

75-90 kDa

Greater than 90% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.