1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Noggin 蛋白, Human (HEK293, His)

Noggin 蛋白, Human (HEK293, His)

目录号: HY-P73322
COA 产品使用指南

Noggin蛋白是一种重要的BMP抑制剂,在神经管、体节生长、软骨形态发生和关节形成中发挥着不可或缺的作用。其同二聚体结构促进与 GDF5 和可能的 GDF6 的显着相互作用,抑制软骨细胞分化。Noggin 蛋白, Human (HEK293, His) 是重组的 头蛋白,由 HEK293 表达,带有 C-His 标签。Noggin 蛋白, Human (HEK293, His) 全长 205 个氨基酸,分子量约为 ~30 kDa。

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规格 价格 是否有货 数量
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2 μg ¥400 In-stock
10 μg ¥1120 In-stock
20 μg ¥1800 In-stock
50 μg ¥3400 In-stock
100 μg   询价  

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描述

Noggin protein is an important BMP inhibitor that plays an indispensable role in the neural tube, somite growth, cartilage morphogenesis, and joint formation. Its homodimeric structure promotes significant interactions with GDF5 and possibly GDF6, inhibiting chondrocyte differentiation. Noggin Protein, Human (HEK293, His) is the recombinant human-derived Noggin protein, expressed by HEK293 , with C-His labeled tag. The total length of Noggin Protein, Human (HEK293, His) is 205 a.a., with molecular weight of ~30 kDa.

研究背景

Noggin 蛋白是骨形态发生蛋白 (BMP) 信号传导复杂领域中的关键抑制剂,在神经管和体节生长中发挥着不可或缺的作用,并有助于软骨形态发生和关节形成的复杂过程。通过其同二聚体结构,Noggin 与 GDF5(可能还有 GDF6)建立了显着的相互作用,对软骨细胞分化发挥抑制作用。这种分子相互作用强调了 Noggin 在调节胚胎发育关键方面的关键地位,强调了它在塑造对正常生长和形态发生至关重要的复杂模式和结构方面的微妙参与。

生物活性

Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.03-0.3 μg/mL in the presence of 25 ng/mL of recombinant human BMP4.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q13253/NP_005441.1 (Q28-C232)

基因 ID
蛋白结构
N-term
Noggin (Q28-C232)
Accession # Q13253/NP_005441.1
His
C-term
同用名
NOG; Noggin; SYM1; SYNS1; SYNS1A
氨基酸序列

QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

分子量

Approximately 30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Noggin Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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