PCSK9 蛋白, Mouse (660a.a, HEK293, His)

The PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2 receptors. It promotes LDLR degradation, inhibits recycling, induces ubiquitination, and affects APOB degradation. Additionally, PCSK9 influences BACE1 intermediates, ENaC surface expression, and neuronal apoptosis by modulating LRP8/APOER2 levels and anti-apoptotic signaling pathways. PCSK9 Protein, Mouse (660a.a, HEK293, His) is the recombinant mouse-derived PCSK9 protein, expressed by HEK293 , with C-His labeled tag. The total length of PCSK9 Protein, Mouse (660a.a, HEK293, His) is 660 a.a., with molecular weight of ~19 & 65 kDa, respectively.

PCSK9 蛋白在血浆胆固醇稳态的复杂调节中发挥着关键作用。它与低密度脂蛋白受体家族的成员结合，包括低密度脂蛋白受体 (LDLR)、极低密度脂蛋白受体 (VLDLR)、载脂蛋白 E 受体 (LRP1/APOER) 和载脂蛋白受体 2 (LRP8/APOER2) ），促进它们在细胞内酸性室内的降解。PCSK9 通过非蛋白水解机制发挥作用，通过网格蛋白 LDLRAP1/ARH 介导的途径增强肝脏 LDLR 的降解，并可能阻碍 LDLR 从内体循环到细胞表面，将其引导至溶酶体进行降解。此外，PCSK9 诱导 LDLR 泛素化，导致随后的降解，并通过自噬体/溶酶体途径抑制 APOB 的细胞内降解，与 LDLR 无关。此外，PCSK9 参与早期分泌途径中 BACE1 非乙酰化中间体的处理，通过增加其蛋白酶体降解来阻碍上皮 Na(+) 通道 (ENaC) 介导的 Na(+) 吸收，并通过调节神经细胞凋亡来调节LRP8/APOER2 水平和相关的抗凋亡信号通路。

Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10 μg/mL (100 μL/well) can bind human LDLR and the EC₅₀ is 0.12 μg/mL.

Mouse

HEK293

C-His

Q80W65 (Q35-Q694)

100102  [NCBI]

MGTHCSAWLRWPLLPLLPPLLLLLLLLCPTGAGAQDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ

Approximately 19 & 65 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 15 mM Tris, 90 mM NaCl, 50% Glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.