1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules Macrophage CD Proteins Monocyte CD Proteins Epithelial cell CD Proteins
  4. PD-L1 PD-L1/CD274
  5. PD-L1 蛋白, Rat (221a.a, HEK293, His)

PD-L1 蛋白, Rat (221a.a, HEK293, His)

目录号: HY-P70814
COA 产品使用指南

PD-L1 蛋白是一种 I 型跨膜蛋白,属于免疫球蛋白 (Ig) 超家族。PD-L1 可以与 PD-1 相互作用,PD-1/PD-L1 轴负责癌症中T细胞的激活、增殖和细胞毒性分泌,并调节抗肿瘤免疫反应。PD-L1 在抑制免疫系统适应性臂方面发挥着重要作用。PD-L1 蛋白, Rat (221a.a, HEK293, His) 是重组的 PD-L1 蛋白,由 HEK293 表达,带有 C-6*His 标签。PD-L1 蛋白, Rat (221a.a, HEK293, His) 全长 221 个氨基酸,分子量为 40-60 kDa。

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10 μg ¥1000 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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描述

PD-L1 Protein is a type I transmembrane protein that belongs to the immunoglobulin (Ig) superfamily. PD-L1 can interact with PD-1, and PD-1/PD-L1 axis is responsible for T cell activation, proliferation, and cytotoxic secretion in cancer, and regulates anti-tumor immune responses. PD-L1 plays a major role in suppressing the adaptive arm of immune systems[1][2][3]. PD-L1 Protein, Rat (221a.a, HEK293, His) is the recombinant rat-derived PD-L1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PD-L1 Protein, Rat (221a.a, HEK293, His) is 221 a.a., with molecular weight of 40-60 kDa.

研究背景

PD-L1 (CD274) 是一种 I 型跨膜蛋白,属于免疫球蛋白 (Ig) 超家族。PD-1 可以与其配体 PD-L1 或 PD-L2 相互作用。PD-1/PD-L1 轴调控癌症中 T 细胞的激活、增殖和细胞毒性分泌,并调节抗肿瘤免疫反应[1]。此外,PD-L1 还可以与 CD80 相互作用,CD80 可以向激活的T细胞传递抑制信号。在 T 细胞中缺乏 PD-1 信号传导的情况下,PD-L1 也能够保护肿瘤细胞免受 I 型和 II 型干扰素的细胞毒性作用以及细胞毒性 T 淋巴细胞 (CTL) 介导的细胞溶解[2]
PD-L1 可以通过维持和增强诱导性 T reg (iT reg) 细胞中 Foxp3 的表达来促进分化,并维持 iT reg 细胞的功能。PD-L1 通过抑制 Akt/mTOR 信号级联反应诱导 iT reg 细胞[3]
PD-L1 通常在巨噬细胞、一些活化的 T 细胞和 B 细胞、DC 和一些上皮细胞表达,尤其是在炎症条件下。值得注意的是,肿瘤细胞表达 PD-L1 可通过“适应性免疫机制”逃避抗肿瘤反应的。因此,PD-L1 通过与其受体结合而在癌细胞中充当促肿瘤因子[1]

种属

Rat

表达系统

HEK293

标签

C-6*His

蛋白编号

D4AE25 (A18-T238)

基因 ID
蛋白结构
N-term
PD-L1 (A18-T238)
Accession # D4AE25
6*His
C-term
同用名
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
氨基酸序列

AFTITAPKDLYVVEYGSNVTMECRFPVEQKLDLLALVVYWEKEDKEVIQFVEGEEDLKPQHSSFRGRAFLPKDQLLKGNAVLQITDVKLQDAGVYCCMISYGGADYKRITLKVNAPYRKINQRISMDPATSEHELMCQAEGYPEAEVIWTNSDHQSLSGETTVTTSQTEEKLLNVTSVLRVNATANDVFHCTFWRVHSGENHTAELIIPELPVPRLPHNRT

分子量

40-60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PD-L1 Protein, Rat (221a.a, HEK293, His)
目录号:
HY-P70814
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