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  3. REG-4 蛋白, Human (HEK293, His)

REG-4 蛋白, Human (HEK293, His)

目录号: HY-P71257
产品使用指南

REG-4 是一种不依赖于钙的凝集素,具有甘露糖结合特异性,即使在酸性环境中也能保留碳水化合物识别活性。它独立于钙发挥作用的能力表明其具有强大且多功能的凝集素活性。REG-4 蛋白, Human (HEK293, His) 是重组的 REG-4 蛋白,由 HEK293 表达,带有 C-6*His 标签。REG-4 蛋白, Human (HEK293, His) 全长 136 个氨基酸,分子量约为 ~16.0 kDa。

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描述

REG-4 is a calcium-independent lectin with mannose-binding specificity that retains carbohydrate recognition activity even in acidic environments. Its ability to act independently of calcium indicates its potent and versatile lectin activity. REG-4 Protein, Human (HEK293, His) is the recombinant human-derived REG-4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of REG-4 Protein, Human (HEK293, His) is 136 a.a., with molecular weight of ~16.0 kDa.

研究背景

REG-4 是一种不依赖于钙的凝集素,对甘露糖具有特定的亲和力,即使在酸性环境中也能保留其碳水化合物识别活性。这表明了一种稳健且适应性强的分子结构,可以在不同的生理条件下有效发挥作用。REG-4 与胃肠道上皮内的炎症和化生反应有关,表明其在调节与炎症和组织转化相关的细胞过程中的潜在作用。REG-4 的甘露糖结合特异性表明它参与识别糖蛋白或其他显示甘露糖残基的分子并与之相互作用,可能有助于胃肠道环境中的免疫反应或细胞信号传导。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9BYZ8 (D23-P158)

基因 ID
蛋白结构
N-term
REG-4 (D23-P158)
Accession # Q9BYZ8
6*His
C-term
同用名
Regenerating islet-derived protein 4; Gastrointestinal secretory protein; REG-like protein; Regenerating islet-derived protein IV; GISP; RELP; REG4
氨基酸序列

DIIMRPSCAPGWFYHKSNCYGYFRKLRNWSDAELECQSYGNGAHLASILSLKEASTIAEYISGYQRSQPIWIGLHDPQKRQQWQWIDGAMYLYRSWSGKSMGGNKHCAEMSSNNNFLTWSSNECNKRQHFLCKYRP

分子量

Approximately 16.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

REG-4 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
REG-4 Protein, Human (HEK293, His)
目录号:
HY-P71257
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