1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. RuvC 蛋白, E.coli (His-SUMO)

RuvC 蛋白, E.coli (His-SUMO)

目录号: HY-P71514
COA 产品使用指南

RuvC 蛋白是 RuvA-RuvB-RuvC 复合物的重要组成部分,对于基因重组和 DNA 修复中霍利迪连接的加工至关重要。作为一种核酸内切酶,RuvC 通过生成依赖于中央同源核心的对称单链切口来解析霍利迪连接。RuvC 蛋白, E.coli (His-SUMO) 是重组的 RuvC 蛋白,由 E. coli 表达,带有 N-SUMO, N-6*His 标签。RuvC 蛋白, E.coli (His-SUMO) 全长 172 个氨基酸,分子量约为 ~34.6 kDa。

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描述

RuvC protein is an important component of the RuvA-RuvB-RuvC complex and is essential for the processing of Holliday junctions in gene recombination and DNA repair. As an endonuclease, RuvC resolves Holliday junctions by generating symmetric single-stranded nicks that rely on a central homology core. RuvC Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RuvC protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of RuvC Protein, E.coli (His-SUMO) is 172 a.a., with molecular weight of ~34.6 kDa.

研究背景

RuvC 蛋白是 RuvA-RuvB-RuvC 复合物的重要组成部分,在基因重组和 DNA 修复过程中霍利迪连接的处理中发挥着至关重要的作用。作为一种核酸内切酶,RuvC 通过在同源臂内对称位置的连接处形成单链切口来解析霍利迪连接体 (HJ) 中间体。这种裂解导致 5'-磷酸基团和 3'-羟基的生成,并且取决于连接处同源中央核心的存在。RuvC 的共有切割序列是 5'-(A/T)TT(C>G/A)-3',切割发生在 TT 二核苷酸 3' 侧的链交换点。值得注意的是,裂解反应可以解偶联,需要存在两个共有裂解序列。RuvC 以序列非特异性方式与十字形 DNA 结合。RuvC 与 RuvA 和 RuvB 结合形成复合物,可提高链交换(分支迁移)的速率,解离 RecA 丝,并促进十字形连接处两个方向的裂解。HJ-RuvA-RuvB-RuvC 复合物不仅能解析霍利迪连接体,而且还能进行分支迁移,证明了分支迁移/HJ 解析反应的耦合。RuvC 的这种综合酶活性强调了它在 DNA 修复和重组的复杂机制中的关键作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

E.coli

表达系统

E. coli

标签

N-SUMO;N-6*His

蛋白编号

P0A814 (A2-R173)

基因 ID

946378  [NCBI]

蛋白结构
N-term
6*His-SUMO
RuvC (A2-R173)
Accession # P0A814
C-term
同用名
ruvC; b1863; JW1852; Crossover junction endodeoxyribonuclease RuvC; EC 3.1.22.4; Holliday junction nuclease RuvC; Holliday junction resolvase RuvC
氨基酸序列

AIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSRLKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEYAARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQMSESRLNLARGRLR

分子量

Approximately 34.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile fltered PBS, 6% Trehalose, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

RuvC Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RuvC Protein, E.coli (His-SUMO)
目录号:
HY-P71514
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