1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E1 Enzymes
  5. SAE1
  6. SAE1 蛋白, Human (GST)

SAE1 蛋白, Human (GST)

目录号: HY-P71643
产品使用指南

SAE1 蛋白形成异二聚体,充当 SUMO1、SUMO2、SUMO3 和潜在的 SUMO4 的 E1 连接酶。它关键介导 SUMO 蛋白的 ATP 依赖性激活,从而能够与 UBA2/SAE2 的活性位点半胱氨酸形成硫酯键。这个过程对于通过苏酰化修饰蛋白质至关重要。SAE1 蛋白, Human (GST) 是重组的 SAE1 蛋白,由 E. coli 表达,带有 N-GST 标签。SAE1 蛋白, Human (GST) 全长 346 个氨基酸,分子量约为 ~65.4 kDa。

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描述

SAE1 protein, forming a heterodimer, acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and potentially SUMO4. It critically mediates ATP-dependent activation of SUMO proteins, enabling the formation of a thioester bond with UBA2/SAE2's active site cysteine. This process is essential for protein modification through sumoylation. SAE1 Protein, Human (GST) is the recombinant human-derived SAE1 protein, expressed by E. coli , with N-GST labeled tag. The total length of SAE1 Protein, Human (GST) is 346 a.a., with molecular weight of ~65.4 kDa.

研究背景

SAE1 蛋白作为异二聚体的一部分发挥作用,充当 SUMO1、SUMO2、SUMO3 和可能的 SUMO4 的 E1 连接酶。它在介导 SUMO 蛋白的 ATP 依赖性激活中发挥着至关重要的作用,促进 SUMO 蛋白与 UBA2/SAE2 上保守活性位点半胱氨酸残基之间随后形成硫酯键。这个过程是通过苏酰化修饰蛋白质不可或缺的一部分。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

Q9UBE0 (M1-K346)

基因 ID
蛋白结构
N-term
GST
SAE1 (M1-K346)
Accession # Q9UBE0
C-term
同用名
Activator of SUMO1; AOS1; HSPC140; Sae1; SAE1_HUMAN; Sentrin/SUMO activating protein AOS1; UBL E1A; UBLE1A
氨基酸序列

MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK

分子量

Approximately 65.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SAE1 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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