1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. T Cell CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Pattern Recognition Receptors
  4. CD283/TLR3 Toll-like Receptor
  5. Toll-like Receptor 3
  6. TLR3 蛋白, Mouse (sf9, His)

TLR3 蛋白, Mouse (sf9, His)

目录号: HY-P73587
产品使用指南

TLR3蛋白是先天性和适应性免疫的重要组成部分,可以识别病原体相关分子模式并激活NF-κ-B、IRF3和细胞因子分泌。配体结合诱导 TLR3 二聚化,有效的信号转导需要沿配体聚集信号单元。TLR3 蛋白, Mouse (sf9, His) 是重组的 TLR3 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。TLR3 蛋白, Mouse (sf9, His) 全长 677 个氨基酸,分子量为 75-80 kDa。

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重组蛋白定制服务

1.  客户无需承担相应的运输费用。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

TLR3 protein is an important component of innate and adaptive immunity and can recognize pathogen-associated molecular patterns and activate NF-κ-B, IRF3 and cytokine secretion. Ligand binding induces TLR3 dimerization, and efficient signal transduction requires clustering of signaling units along the ligand. TLR3 Protein, Mouse (sf9, His) is the recombinant mouse-derived TLR3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of TLR3 Protein, Mouse (sf9, His) is 677 a.a., with molecular weight of 75-80 kDa.

研究背景

TLR3 蛋白是先天性免疫和适应性免疫的关键组成部分,通过识别与微生物相关的特定分子模式,在针对病原体的宿主免疫反应中发挥关键作用。TLR3 被双链 RNA(病毒感染的一个特征)激活,通过接头 TRIF/TICAM1 发挥作用,导致 NF-kappa-B 激活、IRF3 核转位、细胞因子分泌和炎症反应启动。TLR3 以单体和同二聚体形式存在,配体结合触发二聚化。该信号单元包含一个约 40 bp 的 ds-RNA 和两个 TLR3 分子,需要信号单元沿着 ds-RNA 配体横向聚集以实现有效的信号转导。与 UNC93B1、SRC 和 TICAM1 的相互作用,特别是对聚 (I:C) 的反应,有助于其调节功能,突出了 TLR3 介导的免疫反应中涉及的复杂分子机制。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

Q99MB1 (C29-L705)

基因 ID
蛋白结构
N-term
His
TLR3 (C29-L705)
Accession # Q99MB1
C-term
同用名
Toll-like receptor 3; CD283; Tlr3
分子量

75-80 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TLR3 Protein, Mouse (sf9, His)
目录号:
HY-P73587
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