1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Deubiquitinase
  4. UCH Proteins
  5. UCH-L3
  6. UCHL3 蛋白, Mouse (His)

UCHL3 蛋白, Mouse (His)

目录号: HY-P76119
产品使用指南

UCHL3 蛋白是一种泛素羧基末端水解酶,参与细胞过程和蛋白质降解的调节。它特别在睾丸中表达,在精子发生中起着至关重要的作用。UCHL3 蛋白在男性生育能力中的重要性及其作为生殖疾病治疗靶点的潜力使其成为生殖医学领域关注的主题。UCHL3 蛋白, Mouse (His) 是重组的 UCHL3 蛋白,由 E. coli 表达,带有 N-His 标签。UCHL3 蛋白, Mouse (His) 全长 229 个氨基酸,分子量约为 ~30 kDa。

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描述

UCHL3 Protein, a ubiquitin carboxyl-terminal hydrolase, is involved in the regulation of cellular processes and protein degradation. It is particularly expressed in the testes and plays a vital role in spermatogenesis. UCHL3 Protein's significance in male fertility and its potential as a therapeutic target in reproductive disorders make it a subject of interest in reproductive medicine. UCHL3 Protein, Mouse (His) is the recombinant mouse-derived UCHL3 protein, expressed by E. coli , with N-His labeled tag. The total length of UCHL3 Protein, Mouse (His) is 229 a.a., with molecular weight of ~30 kDa.

研究背景

UCHL3 蛋白是一种去泛素化酶 (DUB),通过处理泛素前体和泛素化蛋白,在控制细胞泛素水平方面发挥着至关重要的作用。作为一种硫醇蛋白酶,它特异性识别并水解泛素或 NEDD8 C 端甘氨酸的肽键。UCHL3 蛋白表现出对“Lys-48”连接的泛素链的偏好,并且对位置 P3'' 处的 Arg 和 Lys 具有 10 倍的偏好。其去泛素化活性包括顶端区室中 ENAC 的去泛素化,调节顶膜的回收。此外,UCHL3 蛋白还能间接增强 IGFIR、AKT 和 FOXO1 的磷酸化,从而促进胰岛素信号传导和胰岛素诱导的脂肪形成。它对于压力反应视网膜、骨骼肌和生殖细胞的维持也至关重要。此外,UCHL3 蛋白可能与工作记忆有关,并且可以水解 UBB(+1),UBB(+1) 是泛素的一种突变形式,可抵抗蛋白酶体的降解。

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

Q9JKB1 (E2-A230)

基因 ID

50933  [NCBI]

蛋白结构
N-term
His
UCHL3 (E2-A230)
Accession # Q9JKB1
C-term
同用名
Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; Ubiquitin thioesterase L3
分子量

Approximately 30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UCHL3 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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