1. 重组蛋白
  2. Cytokines and Growth Factors
  3. VEGF & VEGFR
  4. VEGF
  5. VEGF-A
  6. VEGF165 蛋白, zebrafish (sf9)

VEGF165 蛋白, zebrafish (sf9)

目录号: HY-P74476
产品使用指南

VEGF165蛋白是一种重要的生长因子,积极驱动血管生成、血管发生和内皮细胞生长。其多种作用包括诱导内皮细胞增殖、促进迁移、抑制细胞凋亡和增加血管通透性。VEGF165 蛋白, zebrafish (sf9) 是重组的 VEGF165 蛋白,由 Sf9 insect cells 表达,不带标签。VEGF165 蛋白, zebrafish (sf9) 全长 188 个氨基酸,分子量约为 ~22 kDa。

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规格 价格 是否有货
5 μg ¥670 询问价格 & 货期
10 μg ¥1130 询问价格 & 货期
50 μg ¥3180 询问价格 & 货期
100 μg ¥5400 询问价格 & 货期

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描述

The VEGF165 protein is an important growth factor that actively drives vasculogenesis, vasculogenesis, and endothelial cell growth. Its multiple effects include inducing endothelial cell proliferation, promoting migration, inhibiting apoptosis, and increasing vascular permeability. VEGF165 Protein, zebrafish (sf9) is the recombinant VEGF165 protein, expressed by Sf9 insect cells , with tag free. The total length of VEGF165 Protein, zebrafish (sf9) is 188 a.a., with molecular weight of ~22 kDa.

研究背景

VEGF165 蛋白是一种重要的生长因子,积极参与血管生成、血管形成和内皮细胞生长。其多种作用包括诱导内皮细胞增殖、促进细胞迁移、抑制细胞凋亡和诱导血管通透化。VEGF165 既作用于 kdr 和 tie1 上游,刺激内皮细胞分化,又作用于 gata1 上游,刺激造血细胞分化。在结构上,它作为通过二硫键连接的同二聚体存在。同种型 VEGF165 特异性结合 kdr 和 kdrl,突显其在协调对血管发育和维护至关重要的多个细胞过程中的核心作用。

种属

Others

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

O73682 (M1-R188)

基因 ID

30682  [NCBI]

蛋白结构
N-term
VEGF165 (M1-R188)
Accession # O73682
C-term
同用名
VEGF-AA; Vascular endothelial growth factor A-A; vegf; vegfa
分子量

Approximately 22 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

VEGF165 Protein, zebrafish (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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