QL9

目录号: HY-P0287: FAQs
Data Sheet SDS 产品使用指南

摩尔计算器

QL9 是针对2C T细胞受体 (TCR) 的高亲和力同种异体抗原。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Custom Peptide Synthesis

QL9 Chemical Structure

CAS No. : 159646-83-0

1. 客户无需承担相应的运输费用。

2. 同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

可免费申领三个不同产品的试用装。

3. 试用装只面向终端客户。

规格	价格	是否有货
1 mg	￥550	询问价格 & 货期
5 mg	￥1400	询问价格 & 货期
10 mg	￥2100	询问价格 & 货期

or 大包装询价

* Please select Quantity before adding items.

QL9 的其他形式现货产品:

QL9 TFA

Customer Review

Other Forms of QL9:

QL9 TFA In-stock

QL9 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

QL9 (QLSPFPFDL) is a high-affinity alloantigen for the 2C T cell receptor (TCR).

IC₅₀ & Target

TCR^[1]

体外研究
(In Vitro)

Mouse T cell clone 2C recognizes two different major histocompatibility (MHC) ligands, the self MHC K^b and the allogeneic MHC L^d. Two distinct peptides, SIY (SIYRYYGL) and QL9 (QLSPFPFDL), act as strong and specific agonists when bind to K^b and L^d, respectively. QL9 binding to MHC L^d is influenced by the majority of peptide side chains, distributed across the entire length of the peptide. Findings with both systems, but QL9-L^d in particular, suggest that many single-residue substitutions, introduced into peptides to improve their binding to MHC and thus their vaccine potential, could impair T cell reactivity due to their dual impact on TCR binding. T cell activation assays are performed to measure effects of peptide SIY and QL9 residues on T cell function^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1063.20

Formula

C₅₂H₇₄N₁₀O₁₄

CAS 号

159646-83-0

性状

固体

颜色

White to off-white

Sequence

Gln-Leu-Ser-Pro-Phe-Pro-Phe-Asp-Leu

Sequence Shortening

QLSPFPFDL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

纯度 & 产品资料

Data Sheet (518 KB) SDS (252 KB) 产品使用指南 (1538 KB)

参考文献

[1]. Speir JA, et al. Structural basis of 2C TCR allorecognition of H-2Ld peptide complexes. Immunity. 1998 May;8(5):553-62. [Content Brief]

[2]. Bowerman NA, et al. Different strategies adopted by K(b) and L(d) to generate T cell specificity directed against their respective bound peptides. J Biol Chem. 2009 Nov 20;284(47):32551-61. [Content Brief]

Cell Assay ^[2]	Wild type 2C and high affinity 2C T cell transfectants m67 and m6 are incubated with K^b- or L^d-positive cells and various concentrations of peptide SIY and QL9 alanine variants. T cell activation is measured by assaying for levels of IL-2 release. Briefly, T cell transfectants (7.5×10⁴) are incubated with T2-K^b (7.5×10⁴) or T2-L^d (7.5×10⁴) along with various concentrations of peptide for 20-24 h at 37 °C and 5% CO₂. Supernatant is harvested, and levels of IL-2 are measured in an enzyme-linked immunosorbent assay type format. Results are plotted as percentage of maximal IL-2 release=((A_{450 (sample)}-A_{450(no peptide)})/(Max A_450(sample)-A_{450(no peptide)}))×100; signal obtained from no peptide is similar to that obtained for the null peptides MCMV or OVA. Binding curves are generated in GraphPad Prism by plotting the percentage of maximal IL-2 release against peptide concentration. The concentrations of peptide yielding 50% maximal IL-2 release (SD₅₀) are calculated using non-linear regression (sigmoidal fitting; GraphPad Prism) of the activation curves^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Speir JA, et al. Structural basis of 2C TCR allorecognition of H-2Ld peptide complexes. Immunity. 1998 May;8(5):553-62. [Content Brief] [2]. Bowerman NA, et al. Different strategies adopted by K(b) and L(d) to generate T cell specificity directed against their respective bound peptides. J Biol Chem. 2009 Nov 20;284(47):32551-61. [Content Brief]

Cell Assay
^[2]

Wild type 2C and high affinity 2C T cell transfectants m67 and m6 are incubated with K^b- or L^d-positive cells and various concentrations of peptide SIY and QL9 alanine variants. T cell activation is measured by assaying for levels of IL-2 release. Briefly, T cell transfectants (7.5×10⁴) are incubated with T2-K^b (7.5×10⁴) or T2-L^d (7.5×10⁴) along with various concentrations of peptide for 20-24 h at 37 °C and 5% CO₂. Supernatant is harvested, and levels of IL-2 are measured in an enzyme-linked immunosorbent assay type format. Results are plotted as percentage of maximal IL-2 release=((A_{450 (sample)}-A_{450(no peptide)})/(Max A_450(sample)-A_{450(no peptide)}))×100; signal obtained from no peptide is similar to that obtained for the null peptides MCMV or OVA. Binding curves are generated in GraphPad Prism by plotting the percentage of maximal IL-2 release against peptide concentration. The concentrations of peptide yielding 50% maximal IL-2 release (SD₅₀) are calculated using non-linear regression (sigmoidal fitting; GraphPad Prism) of the activation curves^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Speir JA, et al. Structural basis of 2C TCR allorecognition of H-2Ld peptide complexes. Immunity. 1998 May;8(5):553-62. [Content Brief]

[2]. Bowerman NA, et al. Different strategies adopted by K(b) and L(d) to generate T cell specificity directed against their respective bound peptides. J Biol Chem. 2009 Nov 20;284(47):32551-61. [Content Brief]

摩尔计算器
稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量		浓度		体积		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)	×	体积 _(start)	=	浓度 _(final)	×	体积 _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

QL9159646-83-0QL 9QL-9OthersInhibitorinhibitorinhibit

您最近查看的产品:

产品名称：

* 需求量：

* 客户姓名：

* Email：

* 电话：

* 公司或机构名称：

留言给我们：

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。沪(浦)应急管危经许[2021]201709(QFYS)	站点地图隐私声明
Copyright © 2013-2024 MedChemExpress. All Rights Reserved.	沪ICP备15051369号-4

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。沪(浦)应急管危经许[2021]201709(QFYS)

站点地图隐私声明

QL9

Customer Review

Other Forms of QL9:

QL9 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

摩尔计算器

稀释计算器

The molarity calculator equation

The dilution calculator equation

Keywords:

您最近查看的产品:

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

产品名称：			* Lot #：
* 客户姓名：			* Email：
电话：			* 部门：
* 公司或机构名称：
留言给我们：

姓名
邮箱 *

Sorry, but the email address you supplied was invalid.

QL9

Customer Review

Other Forms of QL9:

QL9 相关抗体

QL9 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

QL9 相关抗体:

摩尔计算器

稀释计算器

The molarity calculator equation

The dilution calculator equation

Keywords:

您最近查看的产品:

Request for HNMR Report

Request for HNMR Report

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z