1. Cell Cycle/DNA Damage
  2. CRISPR/Cas9

CRISPR/Cas9

CRISPR/Cas9 (Clustered Regularly-Interspaced Short Palindromic Repeats/Cas9) is a bacterial immune system that has been adapted for genome editing in mammalian cells. Cas9 is a programmable nuclease that generates double-stranded breaks (DSB) in DNA dictated by binding of a ~20 nucleotide recombinant “guide RNA” (gRNA) to the target site. DSB’s produced by Cas9 are most often repaired through the cell’s error-prone non-homologous end joining (NHEJ) pathway, resulting in small insertions or deletions (indels). The vast majority of indels shift the reading frame, introducing a premature stop codon or resulting in nonsense mediated decay of the mRNA-effectively “knocking out” a gene.

The CRISPR/Cas9 system has substantially advanced efforts in specific gene editing and has been successfully applied to modify the episomal genomes of human and other organisms. The CRISPR/Cas9 system utilizes a prokaryotic RNA-guided programmable nuclease that can make a double-strand DNA break (DSB) at a specific site under the guidance of a leading RNA. This DSB process depends on the co-expression of two basic components: a guide RNA (gRNA) and Cas9 nuclease. Making a specific DSB can trigger DNA repair via either error-prone non-homologous end joining (NHEJ) or homology-directed repair (HDR). In the presence of the CRISPR/Cas9 system, the NHEJ inhibitor SCR7 is proven to increase the efficiency of Cas9-mediated HDR by at least by 7-fold in mammalian cells. Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells.

CRISPR/Cas9 相关产品 (6):

Cat. No. Product Name Effect Purity
  • HY-16592
    Brefeldin A Activator 99.54%
    Brefeldin A 是一种真菌代谢物,可以扰乱高尔基体的结构和功能,阻断蛋白的转运,能够增强 CRISPR/Cas9 的活性。
  • HY-13520
    Nocodazole Activator 99.05%
    Nocodazole 是一种快速可逆的 microtubule 聚合抑制剂,也能够有效抑制ABL,ABL(E255K) 和 ABL(T315I) 的活性,IC50 值分别为 0.21 μM,0.53 μM 和 0.64 μM;同时可以增强 CRISPR/Cas9 的活性。
  • HY-12742
    SCR7 Agonist 99.78%
    SCR7 是一种DNA连接酶IV (DNA Ligase IV) 抑制剂,抑制非同源末端连接 (NHEJ)。SCR7 增强CRISPR/Cas9条件的编辑频率。
  • HY-11006
    KU-57788 Activator 98.74%
    KU-57788 是一种有效的,选择性的 DNA-PK 抑制剂,并且适度抑制 BRD4BRDTIC50 值分别为 13 nM,1 μM 和 3.5 μM;同时能够激活 CRISPR/Cas9
  • HY-16950
    4-Hydroxytamoxifen Agonist 99.27%
    4-Hydroxytamoxifen是tamoxifen的活性代谢产物,是一种选择性的雌激素受体调节分子,在乳腺癌的治疗和化学疗法中应用广泛。
  • HY-19793
    RS-1 Activator 99.42%
    RS-1对RAD51有作用,能够增加RAD51结合活性,有报告称RS-1能够杀死癌细胞。