1. Cell Cycle/DNA Damage
  2. DNA Stain
  3. Biotin-16-UTP

Biotin-16-UTP  (Synonyms: Biotin-16-UTP tetralithium)

目录号: HY-D1686
产品使用指南 技术支持

Biotin-16-UTP 是 RNA 聚合酶的活性底物。Biotin-16-UTP 可在体外转录反应中取代 UTP 进行 RNA 标记。

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Biotin-16-UTP Chemical Structure

Biotin-16-UTP Chemical Structure

CAS No. : 186033-13-6

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Biotin-16-UTP is an active substrate for RNA polymerase. Biotin-16-UTP can replace UTP in the in vitro transcription reaction for RNA labeling[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
In Vitro RNA Synthesis and Purification:
1. Incubate the cells according to your normal protocol.
2. Add gently One volume of transcription buffer 2x [200 mM KCI, 20 mM Tris-HCI, pH 8.0, 5 mM MgCl2, 4 mM dithiothreitol (DTT), 4 mM each of ATP, GTP and CTP, 200 mM sucrose and 20% glycerol] to nuclei in ice, form mixture.
3. Add 8 μL biotin-16-UTP (from 10 mM tetralithium sal) to the mixture, which is incubated for 30 min at 29°C.
4. Add 6 μL 250 mM CaCl2, 6 μL RNase-free DNase I (10 U/μL) and incubating for 10 min at 29°C to stop reaction.
5. Perform RNA purification of both nuclear run-on and total RNA according to the manufacturer's instructions.
6. Resuspend RNA in 50 uL diethylpyrocarbonate (DEPC)-treated water.
7. Labeled RNA was captured by streptavidin-coated magnetic beads.
8. RNA-binding beads are then used for random hexamer primed reverse transcription.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

987.51

同用名

Biotin-16-UTP tetralithium

Formula

C32H48Li4N7O19P3S

CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HY-D1686
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