CellTracker Red CMTPX 

CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm)^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fixation and staining of self-assembled spheroids^[1]:
1. Resuspended cells are stained with CellTracker Red CMTPX and allowed to self-assemble into spheroids before aggregation.
2. Cell aggregates are fixed in 4% paraformaldehyde/PBS for 30 min with gentle inversion, then washing cells in PBS for 3 times and stained with 100 ng/mL Hoechst dye overnight prior to imaging.

使用指南 (以下是我们推荐的方案，本方案仅提供指南，应根据您的具体需要进行修改)。
自组装球体的固定和染色^[1]:
1. 用 CellTracker Red CMTPX 对重悬的细胞进行染色，并允许其在聚集之前自组装成球体。
2. 将细胞聚集物在 4% 多聚甲醛/PBS 中固定 30 分钟，温和倒置，然后在 PBS 中洗涤细胞 3 次，用 100 ng/mL 的 Hoechst 染料染色过夜，然后进行成像。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

686.24

C₄₂H₄₀ClN₃O₄

油状物

Blue to dark blue

614

586

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Van Pel DM, et al. Modelling glioma invasion using 3D bioprinting and scaffold-free 3D culture. J Cell Commun Signal. 2018 Dec;12(4):723-730.  [Content Brief]