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  3. Desthiobiotin-PEG3-NHS ester

Desthiobiotin-PEG3-NHS ester 是一种单环无硫生物素类似物,它与链霉亲和素的结合特异性几乎相同,但亲和力低于生物素 (Kd = 1011 与 1015 M)。因此,脱硫生物素化的蛋白质可以在基于与游离生物素竞争置换的温和条件下,从链霉亲和素亲和树脂中轻松且特异性地洗脱。

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Desthiobiotin-PEG3-NHS ester Chemical Structure

Desthiobiotin-PEG3-NHS ester Chemical Structure

CAS No. : 2411681-92-8

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  • 生物活性

  • 纯度 & 产品资料

生物活性

Desthiobiotin-PEG3-NHS ester is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin.

分子量

514.57

Formula

C23H38N4O9

CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

Desthiobiotin-PEG3-NHS ester 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Desthiobiotin-PEG3-NHS ester
目录号:
HY-W190908
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