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IR 813 perchlorate  (Synonyms: IR-813 高氯酸盐)

目录号: HY-D1535
产品使用指南

IR 813 perchlorate 是一种近红外 (NIR) 荧光染料 (Ex=815 nm,Em=840 nm),可用于对小鼠的局部淋巴结进行可视化。

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IR 813 perchlorate Chemical Structure

IR 813 perchlorate Chemical Structure

CAS No. : 201024-57-9

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IR 813 perchlorate 的其他形式现货产品:

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

IR 813 perchlorate is a near-infrared (NIR) fluorescent dye (Ex=815 nm, Em=840 nm) and can be used for visualizing regional lymph nodes in mice[1].

体外研究
(In Vitro)

IR 813 (17.3 μM, 2 h) induces a 31.4% hemolysis in red blood cells[1].
IR 813 (5.9 μM, 24 h) induces 50% cell death in MRC-5 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

IR 813 perchlorate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.
2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.
3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).
4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

683.66

Formula

C40H40Cl2N2O4

CAS 号
Emission (Em)

813

Excitation (Ex)

780

中文名称

IR-813 高氯酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IR 813 perchlorate
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