ADAM10 蛋白, Human (HEK293, His)

The ADAM10 protein cleaves TNF-α precursor, releasing a soluble form, and mediates the proteolytic release of JAM3 and various cell surface proteins. ADAM10 Protein, Human (HEK293, His) is the recombinant human-derived ADAM10 protein, expressed by HEK293 , with C-10*His labeled tag.

ADAM10 蛋白是一种具有广泛底物特异性的多面蛋白酶，在多种生物过程中发挥着核心作用。值得注意的是，ADAM10 裂解 TNF-α 的膜结合前体，释放其成熟的可溶形式。此外，它还负责各种细胞表面蛋白的蛋白水解释放，包括 JAM3、肝素结合表皮生长样因子、肝配蛋白-A2、CD44、CDH2 以及淀粉样前体蛋白的组成型和调节型 α 分泌酶裂解。应用程序）。ADAM10 有助于细胞朊病毒蛋白的正常裂解，并参与 L1 等粘附分子的裂解。此外，它控制 Notch 的蛋白水解加工，介导神经发生过程中的侧向抑制，并负责 FasL 胞外域脱落。ADAM10 在介导各种整合膜蛋白（包括 CORIN、ITM2B、LAG3、IL6R、IL11RA、CHL1、NRCAM 和 TREM2）的蛋白水解裂解中发挥多种作用。此外，ADAM10 在肾小球和冠状脉管系统的发育和成熟中发挥着至关重要的作用，有助于 Corti 耳蜗发育过程中的支柱细胞分离，并调节 EFNA5-EPHA3 信号传导。在微生物感染中，ADAM10通过与粘附小带的毒素结合并促进毒素孔的形成来促进金黄色葡萄球菌的细胞毒活性。

Measured by its ability to cleave 10 µM fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (HY-P4931).. The specific activity is >20 pmol/min/µg.

Human

HEK293

C-10*His

O14672 (T214-E672)

102

Extracellular Domain (with Propeptide)

Approximately 60-71 kDa, due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution in 20 mM MES, 300 mM NaCl, pH 5.5, 10 mM ZnCl₂, 0.1% Brij-35, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.