Alkaline Phosphatase/ALPP 蛋白, Human (HEK293, C-His)

Alkaline Phosphatase/ALPP Proteinas, an enzyme, effectively hydrolyzes various phosphate compounds. Alkaline Phosphatase/ALPP Protein, Human (HEK293, C-His) is the recombinant human-derived Alkaline Phosphatase/ALPP protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Alkaline Phosphatase/ALPP Protein, Human (HEK293, C-His) is 484 a.a., with molecular weight of approximately 62.5 kDa.

碱性磷酸酶（ALP）由 ALPP 基因编码，是一种以在碱性条件下有效水解各种磷酸盐化合物而闻名的酶。这种蛋白质在维持磷酸盐稳态中发挥着至关重要的作用，并参与多种生理过程。ALP 主要由骨组织中的成骨细胞表达，对于骨形成过程中的矿化至关重要。此外，ALP 作为一种有价值的生物标志物，特别是在评估肝脏和骨骼健康方面，血清水平升高表明某些疾病。

Immobilized  Human ALPP at 1 μg/mL (100 μL/well) can bind Anti-ALPP Antibody, The ED₅₀ for  this effect is 3.432 ng/mL.

Human

HEK293

C-6*His

P05187 (I23-D506)

250  [NCBI]

IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDHHHHHH

approximately 62.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.