AlkB 蛋白, E.coli (Myc, His-SUMO)

AlkB Protein, E.coli (Myc, His-SUMO) is a protein found in E. coli, plays a major role in oxidative dealkylation processes.

AlkB 蛋白家族的铁依赖性双加氧酶存在于整个生命树的大多数生物体中，在氧化脱烷基化过程中发挥着重要作用。在包括酵母、植物和哺乳动物在内的高等真核生物中，AlkB 家族的成员发挥着关键作用。在人类中，该家族因其参与 DNA 修复和癌症进展而受到特别关注，因为它们经常在肿瘤中过度表达并抵消化疗中使用的替莫唑胺等烷化药物的作用。大肠杆菌 alkB 突变体的烷基化敏感表型标志着 alkB 途径是一种极其有效的防御机制，可对抗 SN2（而非 SN1）烷化剂的细胞毒性作用^[1][2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-His;C-Myc;N-SUMO

P05050 (1M-216E)

946708  [NCBI]

MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQAGKKE

Approximately 44.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. V Van Deuren, et al. Structural determinants of nucleobase modification recognition in the AlkB family of dioxygenases. DNA Repair (Amst). 2020 Dec;96:102995.  [Content Brief]

  [2]. B J Chen, et al. The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity. J Bacteriol. 1994 Oct;176(20):6255-61.  [Content Brief]