Animal-Free IGF-II 蛋白, Mouse (His)

The IGF-II protein plays a crucial role in glucose-mediated insulin secretion, acting together with insulin as a physiological amplifier. Furthermore, it exhibits osteogenic properties by enhancing osteoblast mitotic activity through phosphorylation of MAPK1 and MAPK3. Animal-Free IGF-II Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIGF-II protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free IGF-II Protein, Mouse (His) is 67 a.a., with molecular weight of ~8.20 kDa.

IGF-II 蛋白是葡萄糖介导的胰岛素分泌的关键因素，与胰岛素共同分泌，并充当该过程的生理放大器。值得注意的是，IGF-II 通过磷酸化 MAPK1 和 MAPK3 增强成骨细胞有丝分裂活性，从而表现出成骨特性。这种双重作用强调了该蛋白质在葡萄糖稳态和骨代谢中的重要性，强调了其在与胰岛素分泌和骨健康相关的背景下的潜在治疗意义。

Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is <6 ng/mL. The specific activity of recombinant mouse IGF-II is >1.5 x 10⁵ IU/mg.

Mouse

E. coli

N-His

D3Z4N4 (A25-E91)

16002

AYGPGETLCGGELVDTLQFVCSDRGFYFSRPSSRANRRSRGIVEECCFRSCDLALLETYCATPAKSE

Approximately 8.20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.