Animal-Free IL-1 alpha 蛋白, Mouse (His)

IL-1 alpha protein is an important cytokine found intracellularly in non-hematopoietic cells that bridges innate and adaptive immunity. Binds to IL1R1 and IL1RAP to form a high-affinity receptor complex, initiates signaling through MYD88, IRAK1 or IRAK4, and activates the NF-kappa-B and MAPK pathways. Animal-Free IL-1 alpha Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-1 alpha protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-1 alpha Protein, Mouse (His) is 156 a.a., with molecular weight of ~18.93 kDa.

细胞因子白细胞介素 1 α (IL-1 α) 基本存在于几乎所有静止非造血细胞的细胞内，在炎症中发挥着至关重要的作用，并充当先天性免疫系统和适应性免疫系统之间的桥梁。与其受体 IL1R1 结合后，与其辅助蛋白 IL1RAP 结合，形成高亲和力的 IL1R1 受体复合物。随后的信号转导事件涉及 MYD88、IRAK1 或 IRAK4 等接头分子的募集，从而导致 NF-kappa-B 和三个 MAPK 通路（p38、p42/p44 和 JNK 通路）的激活。在细胞内，IL-1 α 起到警报素的作用，细胞死亡后，它会在细胞膜破裂后释放到细胞外空间，诱导炎症并发出宿主对损伤或损害的反应信号。除了在细胞坏死期间释放危险信号之外，IL-1 α 还可以直接感知 DNA 损伤，作为基因毒性应激的信号，而不损害细胞完整性。此外，IL-1 α 与多种蛋白质（包括 TMED10）相互作用，促进从细胞质易位至内质网-高尔基中间室 (ERGIC) 以及随后的分泌。它与 IL1R1 和 S100A13 的相互作用进一步促进了其复杂的调节机制，后者是 IL-1 α 输出的关键步骤，涉及复合物跨质膜的直接易位。

Measure by its ability to induce D10.G4.1 cells proliferation. The ED₅₀ for this effect is <5 pg/mL. The specific activity of recombinant mouse IL-1 alpha is > 2 x 10⁸ IU/mg.

Mouse

E. coli

C-His

P01582 (S115-S270)

16175  [NCBI]

MSAPYTYQSDLRYKLMKLVRQKFVMNDSLNQTIYQDVDKHYLSTTWLNDLQQEVKFDMYAYSSGGDDSKYPVTLKISDSQLFVSAQGEDQPVLLKELPETPKLITGSETDLIFFWKSINSKNYFTSAAYPELFIATKEQSRVHLARGLPSMTDFQIS

Approximately 18.93 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.