Animal-Free IL-12 beta 蛋白, Mouse (His)

IL-12 beta protein is a cytokine that acts as a growth factor for activated T cells and NK cells, enhances lytic activity and stimulates IFN-γ production. It combines with IL23A to form IL-23, a cytokine critical in innate and adaptive immunity. Animal-Free IL-12 beta Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-12 beta protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-12 beta Protein, Mouse (His) is 313 a.a., with molecular weight of ~36.60 kDa.

IL-12 β 蛋白是一种细胞因子，可作为激活的 T 细胞和 NK 细胞的生长因子，增强 NK/淋巴因子激活的杀伤细胞的裂解活性，并刺激静息 PBMC 产生 IFN-γ。此外，它与 IL23A 结合形成 IL-23 白细胞介素，这是一种异二聚体细胞因子，在先天免疫和适应性免疫中发挥着至关重要的作用。当 IL-23 与由 IL12RB1 和 IL23R 组成的异二聚体受体复合物结合时，会激活 Jak-Stat 信号级联，优先刺激记忆 T 细胞而不是幼稚 T 细胞，并促进促炎细胞因子的产生。这种白细胞介素可能与 IL-17 一起构成对外周组织感染的急性反应。然而，IL-23 的参与延伸到诱导自身免疫性炎症，可能导致自身免疫性炎症疾病，并且它可能在肿瘤发生中发挥重要作用。

Measure by its ability to induce proliferation in T-cell enriched PBMC. The ED₅₀ for this effect is <0.3 ng/mL.

Mouse

E. coli

C-His

P43432 (M23-S335)

16160  [NCBI]

MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

Approximately 36.60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.