Animal-Free IP-10/CRG-2/CXCL10 蛋白, Mouse (His)

The IP-10/CRG-2/CXCL10 Protein, a pro-inflammatory cytokine, modulates immune cells, cell growth, apoptosis, and angiostatic effects. Crucial during viral infections, it activates and migrates immune cells to infected sites by binding to CXCR3. Mechanistically, it triggers G protein-mediated signaling, activating the phospholipase C-dependent pathway, increasing intracellular calcium, and reorganizing actin. This axis also aids neuronal response to brain injury by activating microglia. The IP-10/CRG-2/CXCL10 Protein can exist as a monomer, dimer, or tetramer and interacts with the CXCR3 receptor. Animal-Free IP-10/CRG-2/CXCL10 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIP-10/CRG-2/CXCL10 protein, expressed by E. coli, with N-His labeled tag. The total length of Animal-Free IP-10/CRG-2/CXCL10 Protein, Mouse (His) is 77 a.a., with molecular weight of ~9.47 kDa.

IP-10/CRG-2/CXCL10 蛋白是一种促炎细胞因子，参与免疫细胞的趋化、分化和激活等多种过程，以及细胞生长、凋亡的调节和血管抑制的调节影响。它通过刺激免疫细胞的激活和迁移到感染部位，在病毒感染过程中发挥至关重要的作用。从机制上讲，它与 CXCR3 受体结合，激活 G 蛋白介导的信号传导，并导致磷脂酶 C 依赖性途径的激活、细胞内钙的产生增加和肌动蛋白重组。CXCL10/CXCR3 轴的激活对于神经元响应脑损伤也很重要，因为它激活小胶质细胞并将其引导至损伤部位，促进神经元重组。IP-10/CRG-2/CXCL10 蛋白可以单体、二聚体或四聚体形式存在，并与 CXCR3 受体相互作用。

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR3. The ED₅₀ for this effect is <0.2 μg/mL

Mouse

E. coli

N-His

P17515 (I22-P98)

15945  [NCBI]

IPLARTVRCNCIHIDDGPVRMRAIGKLEIIPASLSCPRVEIIATMKKNDEQRCLNPESKTIKNLMKAFSQKRSKRAP

Approximately 9.47 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.