Animal-Free MCP-1/CCL2 蛋白, Human (His)

MCP-1/CCL2, Human, acts as a ligand for CCR2, eliciting a potent chemotactic response and intracellular calcium mobilization upon CCR2 binding. It specifically attracts monocytes and basophils while sparing neutrophils and eosinophils. Implicated in atherosclerosis, MCP-1/CCL2 may recruit monocytes to arterial walls. Presenting as a monomer or homodimer in equilibrium, it binds to endothelial cells via glycosaminoglycan (GAG) side chains and interacts with TNFAIP6 through its Link domain. Animal-Free MCP-1/CCL2 Protein, Human (His) is the recombinant human-derived animal-FreeMCP-1/CCL2 protein, expressed by E. coli, with N-His labeled tag. The total length of Animal-Free MCP-1/CCL2 Protein, Human (His) is 76 a.a., with molecular weight of ~9.49 kDa.

MCP-1/CCL2（人）作为 CC 趋化因子受体 CCR2 的配体，在 CCR2 结合和激活后启动强大的趋化反应和细胞内钙动员。该趋化因子表现出特异性针对单核细胞和嗜碱性粒细胞的趋化活性，将中性粒细胞或嗜酸性粒细胞排除在其影响之外。此外，MCP-1/CCL2 可能在动脉粥样硬化进展过程中将单核细胞募集到动脉壁中发挥作用。它以单体或同型二聚体平衡存在，通过蛋白聚糖的糖胺聚糖 (GAG) 侧链锚定于内皮细胞。此外，MCP-1/CCL2 通过其 Link 结构域与 TNFAIP6 相互作用。

Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A. The ED₅₀ for this effect is <20 ng/mL.

Human

E. coli

N-His

P13500 (Q24-T99)

6347  [NCBI]

QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

Approximately 9.49 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.