Annexin A1/ANXA1 蛋白, Mouse (His)

Annexin A1 (ANXA1) plays multifaceted roles in innate and adaptive immune responses. It acts as an effector of glucocorticoid-mediated responses, downregulating early inflammation. Annexin A1/ANXA1 Protein, Mouse (His) is the recombinant mouse-derived Annexin A1/ANXA1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Annexin A1/ANXA1 Protein, Mouse (His) is 345 a.a., with molecular weight of ~42.6 kDa.

膜联蛋白 A1 (ANXA1) 在先天免疫反应中扮演着多面角色，既充当糖皮质激素介导反应的效应器，又充当炎症过程的调节器。ANXA1 具有强大的抗炎活性，有助于糖皮质激素介导的炎症反应早期阶段的下调。除了在先天免疫系统中的作用之外，ANXA1 通过增强 T 细胞激活触发的信号级联，从而调节激活 T 细胞的分化和增殖，从而显着影响适应性免疫反应。它促进 T 细胞分化为 Th1 细胞，同时负向调节 T 细胞分化为 Th2 细胞。此外，ANXA1 通过激活甲酰肽受体和重组肌动蛋白细胞骨架，在激素胞吐调节中发挥关键作用。ANXA1 对 Ca(2+) 具有高亲和力，可结合多达 8 个 Ca(2+) 离子，并表现出与磷脂膜的 Ca(2+) 依赖性相互作用。在功能上，ANXA1 有助于吞噬杯和吞噬体的形成，通过介导吞噬体和肌动蛋白细胞骨架之间的 Ca(2+) 依赖性相互作用，在吞噬作用中发挥关键作用。此外，ANXA1通过甲酰肽受体的激活促进粒细胞和单核细胞的趋化性，导致肌动蛋白细胞骨架的重排、细胞极化和细胞迁移。除了其免疫功能外，ANXA1 在炎症消退和伤口愈合中发挥着至关重要的作用，通过中性粒细胞 N-甲酰肽受体发挥作用，增强 CXCL2 的释放。

Mouse

E. coli

N-6*His

P10107 (A2-N346)

16952  [NCBI]

AMVSEFLKQARFLENQEQEYVQAVKSYKGGPGSAVSPYPSFNVSSDVAALHKAIMVKGVDEATIIDILTKRTNAQRQQIKAAYLQENGKPLDEVLRKALTGHLEEVVLAMLKTPAQFDADELRGAMKGLGTDEDTLIEILTTRSNEQIREINRVYREELKRDLAKDITSDTSGDFRKALLALAKGDRCQDLSVNQDLADTDARALYEAGERRKGTDVNVFTTILTSRSFPHLRRVFQNYGKYSQHDMNKALDLELKGDIEKCLTTIVKCATSTPAFFAEKLYEAMKGAGTRHKALIRIMVSRSEIDMNEIKVFYQKKYGISLCQAILDETKGDYEKILVALCGGN

Approximately 42.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.