Apolipoprotein H/APOH 蛋白, Rat (HEK293, His)

Apolipoprotein H/APOH proteins have multiple functions and can bind to negatively charged substances such as heparin and phospholipids. Its diverse interactions highlight the adaptability that is critical for preventing activation of the intrinsic coagulation cascade by binding to phospholipids on the surface of damaged cells. Apolipoprotein H/APOH Protein, Rat (HEK293, His) is the recombinant rat-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag. The total length of Apolipoprotein H/APOH Protein, Rat (HEK293, His) is 345 a.a., with molecular weight of 45-70 kDa.

载脂蛋白 H/APOH 蛋白表现出多种功能，因为它可以与各种带负电的物质结合，包括肝素、磷脂和硫酸葡聚糖。它与这些物质的相互作用表明了广泛的分子参与，突出了它的适应性。值得注意的是，载脂蛋白 H/APOH 通过与受损细胞表面的磷脂特异性结合，在防止内在凝血级联的激活中发挥着至关重要的作用。这一功能强调了其对凝血过程调节的潜在贡献及其在响应细胞损伤时维持止血平衡的重要性。

Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED₅₀ for this effect is 0.7041 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED₅₀ for this effect is 0.7041 μg/mL.

Rat

HEK293

C-6*His

Q5I0M1 (G20-C345)

287774  [NCBI]

GRTCPKPDELPFAVVVPLKTFYDPGEQIVYSCKPGYVSRGGMRRFTCPLTGMWPINTLKCIPRVCPFAGILENGVVRYTTFEYPNTIGFACNPGYYLNGTSSSKCTEEGKWSPELPVCARITCPPPPIPKFAALKEYKTSVGNSSFYQDTVVFKCLPHFAMFGNDTVTCTAHGNWTQLPECREVKCPFPSRPDNGFVNYPAKPVLSYKDKAVFGCHETYKLDGPEEVECTKTGNWSALPSCKASCKLSVKKATVLYQGQRVKIQDQFKNGMMHGDKVHFYCKNKEKKCSYTEEAQCIDGTIEIPKCFKEHSSLAFWKTDASDVTPC

Approximately 45-70 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.