Arginase-1/ARG1 蛋白, Human (N-His)

Arginase-1 (ARG1) protein, crucial in the urea cycle, converts L-arginine to urea and L-ornithine. ARG1 also regulates L-arginine levels outside the liver, impacting immune responses. ARG1's release by granulocytes suppresses T cell and NK cell functions. In ILC2s, ARG1 promotes lung inflammation. However, ARG1's role in human monocytic/macrophage/dendritic cells is unclear. Arginase-1/ARG1 Protein, Human (N-His) is the recombinant human-derived Arginase-1/ARG1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Arginase-1/ARG1 Protein, Human (N-His) is 322 a.a., with molecular weight of ~40 kDa.

精氨酸酶-1 (ARG1) 蛋白是尿素循环的重要组成部分，促进 L-精氨酸转化为尿素和 L-鸟氨酸。该循环主要发生在肝脏中，并且在较小程度上发生在肾脏中。除了在氮代谢中的作用之外，ARG1 在非肝组织中的 L-精氨酸稳态中也发挥着关键作用，它与一氧化氮合酶 (NOS) 竞争细胞内精氨酸，影响先天性和适应性免疫反应。多形核粒细胞中的抗菌效应途径涉及细胞死亡时释放的 ARG1，它会消耗微环境中的精氨酸，导致 T 细胞和自然杀伤 (NK) 细胞增殖和细胞因子分泌受到抑制。在第 2 组先天淋巴细胞 (ILC2) 中，ARG1 促进肺部急性 2 型炎症，影响 ILC2 增殖。然而，ARG1 在人类单核细胞/巨噬细胞/树突状细胞谱系中的精确免疫学作用仍不确定。

Measured by its binding ability in the production of urea during the hydrolysis of arginine. The specific activity is 129570.707 pmol/min/µg.

Human

E. coli

N-6*His

P05089 (M1-K322)

383  [NCBI]

MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK

Approximately 40 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.