BDNF 蛋白, Human

Brain Derived Neurotrophic Factor (BDNF) is a neurotrophin that belongs to NGF-beta family. BDNF can bind to its high affinity receptor TrkB and activates signal transduction cascades (IRS1/2, PI3K, Akt)^[1]. BDNF can also bind to the p75NTR, but the affinity for the p75NTR receptor is lower than for TrkB^[2]. BDNF is a neurotransmitter modulator, and is vital in maturation, survival and differentiation of neuronal populations during development. BDNF also participates in neuronal plasticity, which is essential for learning and memory. BDNF is widely expressed in the CNS^[1]. BDNF Protein, Human is a recombinant human BDNF (H129-R247) without tag, which is produced in E.coli.

BDNF，一种神经营养因子，属于 NGF-β 家族。BDNF 广泛表达于中枢神经系统、肠道和其他组织中。BDNF 调节神经发育过程，包括神经元群体的成熟、存活和分化以及突触可塑性^[1]。
BDNF 可以与其高亲和力受体 TrkB 结合并激活信号转导级联（IRS1/ 2、PI3K、Akt），从而诱导 Ca²⁺ 摄入增加和转录因子磷酸化。BDNF也可以与p75NTR结合，但对p75NTR受体的亲和力低于对TrkB的亲和力。p75NTR 的激活分别通过激活 c-Jun N 末端激酶 (JNK) 和 NF-κB 表达来增加神经元和神经胶质细胞中的凋亡和炎症信号传导^[2]。在人类中，BDNF 水平降低与神经退行性疾病（例如帕金森病和阿尔茨海默病）和 2 型糖尿病^[1]有关。人类 BDNF 与小鼠和大鼠的氨基酸序列同一性超过 97%。大鼠 BDNF 与小鼠的氨基酸序列一致性 >99%。
BDNF 是一种神经递质调节剂，对于发育过程中神经元群的成熟、存活和分化至关重要。BDNF 还参与神经元可塑性，这对于学习和记忆至关重要^[1]。

BDNF (重组人源蛋白, 50 ng/mL, 3 天) 增加 PC12 细胞中的神经突细胞数目百分比^[3]。

BDNF (人，0.25 μg/侧，海马体内给药) 增加大鼠的 ERK1/2 和 CREB 激活，并促进 LTM^[4]。

1.Immobilized Human TrkB-His at 5 μg/mL (100 μl/well) can bind Human BDNF* Biotinylated by NHS-biotin prior to testing. The ED₅₀ of Human BDNF* is ≤6 ng/mL.
2.Immobilized Human TrkB-His at 2 μg/mL (100 μl/well) can bind Human BDNF* Biotinylated by NHS-biotin prior to testing. The ED₅₀ of Human BDNF* is <100 ng/mL.

Human

E. coli

Tag Free

P23560 (H129-R247)

627  [NCBI]

HSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Approximately 13 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, 250 mM NaCl, pH 7.2.

<0.01 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Bathina S, et al. Brain-derived neurotrophic factor and its clinical implications. Arch Med Sci. 2015 Dec 10;11(6):1164-78.  [Content Brief]

  [2]. Li M, et al. Recombinant human brain-derived neurotrophic factor prevents neuronal apoptosis in a novel in vitro model of subarachnoid hemorrhage. Neuropsychiatr Dis Treat. 2017 Apr 3;13:1013-1021.  [Content Brief]

  [3]. Lima Giacobbo B, et al. Brain-Derived Neurotrophic Factor in Brain Disorders: Focus on Neuroinflammation. Mol Neurobiol. 2019 May;56(5):3295-3312.  [Content Brief]

  [4]. Hang P, et al. Brain-derived neurotrophic factor attenuates doxorubicin-induced cardiac dysfunction through activating Akt signalling in rats. J Cell Mol Med. 2017 Apr;21(4):685-696.  [Content Brief]

  [5]. Egan MF, et al. The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function. Cell. 2003 Jan 24;112(2):257-69.  [Content Brief]

  [6]. Alonso M, et al. Signaling mechanisms mediating BDNF modulation of memory formation in vivo in the hippocampus. Cell Mol Neurobiol. 2002 Dec;22(5-6):663-74.  [Content Brief]