BIRC5 蛋白, Human

BIRC5 Protein, Human, a protein in the intrinsic apoptotic pathway that interacts with XIAP and DIABLO leading to caspase-3 and caspase-9 inactivation. BIRC5 (survivin) is also involved in stabilizing the microtubule-kinetochore dynamics^[1].

与正常组织、成人恶性肿瘤和非恶性胎儿肾上腺神经母细胞相比，BIRC5 表达在神经母细胞瘤中显着上调^[1]。
IRC5/Survivin 通过改变对有氧糖酵解的调节来增强有氧糖酵解和耐药性。线粒体融合/裂变机制^[2]。

Human

E. coli

Tag Free

O15392 (M1-D142)

332  [NCBI]

MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD

16-18 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM Tris, 0.1M NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Fieke Lamers, et al. Knockdown of survivin (BIRC5) causes apoptosis in neuroblastoma via mitotic catastrophe. Endocr Relat Cancer. 2011 Oct 27;18(6):657-68.  [Content Brief]

  [2]. J Hagenbuchner, et al. BIRC5/Survivin enhances aerobic glycolysis and drug resistance by altered regulation of the mitochondrial fusion/fission machinery. Oncogene. 2013 Oct;32(40):4748-57.  [Content Brief]