1. 重组蛋白
  2. Complement System
  3. Complement Component 6
  4. C6/Complement component C6 蛋白, Human (HEK293, His)

C6/Complement component C6 蛋白, Human (HEK293, His)

目录号: HY-P74373
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C6/Complement component C6 蛋白补体激活过程中形成的裂解膜攻击复合物的一部分,通过在靶细胞质膜上形成孔,在先天性和获得性免疫反应以及癌症免疫监视中发挥着不可或缺的作用。它是补体级联的一个组成成分,可以融入细胞膜并引起细胞裂解。C6缺乏症患者容易受到细菌感染。C6/Complement component C6 蛋白, Human (HEK293, His) 是重组的 C6/补体成分 C6 蛋白,由 HEK293 表达,带有 C-His 标签。

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5 μg ¥300 In-stock
10 μg ¥480 In-stock
50 μg ¥1250 In-stock
100 μg ¥2000 In-stock
500 μg ¥5600 In-stock
1 mg ¥8900 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

C6/Complement component C6 Protein is a part of the lytic membrane attack complex formed during complement activation, which has an indispensable role in innate and acquired immune responses by forming pores in the plasma membrane of target cells, as well as cancer immune surveillance. It is a component of the complement cascade which can be incorporated into the cell membrane and cause cell lysis. People with C6 deficiency are prone to bacterial infection. C6/Complement component C6 Protein, Human (HEK293, His) is the recombinant human-derived C6/Complement component C6 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

补体成分C6 (C6) 是补体激活过程中形成的裂解膜攻击复合物的一部分,通过在靶细胞质膜上形成孔,在先天性和获得性免疫反应以及癌症免疫监视中发挥着不可或缺的作用。它是补体级联的一个组成成分,可以融入细胞膜并引起细胞裂解。纯化的人 C6 是末端补体途径所特有的,并且在炎症反应中也发挥着关键作用。C6 缺乏症是最常见的终末补体成分缺乏症,可在年龄较大时出现脑膜炎奈瑟菌感染[1][2]

生物活性

Measured by its ability to inhibit the proliferation of HepG2 cells. The ED50 for this effect is 4.961 μg/mL, corresponding to a specific activity is 201.57 units/mg.

  • Measured by its ability to inhibit the proliferation of HepG2 cells. The ED50 for this effect is 4.961 μg/mL, corresponding to a specific activity is 201.57 units/mg.
种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

AAA59668 (C22-A934)

基因 ID

729  [NCBI]

蛋白结构
N-term
C6 (C22-A934)
Accession # AAA59668
His
C-term
蛋白长度

Full Length of Mature Protein

中文名
C6 蛋白/补体成分 C6 蛋白
同用名
Complement component C6; C6
氨基酸序列

CFCDHYAWTQWTSCSKTCNSGTQSRHRQIVVDKYYQENFCEQICSKQETRECNWQRCPINCLLGDFGPWSDCDPCIEKQSKVRSVLRPSQFGGQPCTEPLVAFQPCIPSKLCKIEEADCKNKFRCDSGRCIARKLECNGENDCGDNSDERDCGRTKAVCTRKYNPIPSVQLMGNGFHFLAGEPRGEVLDNSFTGGICKTVKSSRTSNPYRVPANLENVGFEVQTAEDDLKTDFYKDLTSLGHNENQQGSFSSQGGSSFSVPIFYSSKRSENINHNSAFKQAIQASHKKDSSFIRIHKVMKVLNFTTKAKDLHLSDVFLKALNHLPLEYNSALYSRIFDDFGTHYFTSGSLGGVYDLLYQFSSEELKNSGLTEEEAKHCVRIETKKRVLFAKKTKVEHRCTTNKLSEKHEGSFIQGAEKSISLIRGGRSEYGAALAWEKGSSGLEEKTFSEWLESVKENPAVIDFELAPIVDLVRNIPCAVTKRNNLRKALQEYAAKFDPCQCAPCPNNGRPTLSGTECLCVCQSGTYGENCEKQSPDYKSNAVDGQWGCWSSWSTCDATYKRSRTRECNNPAPQRGGKRCEGEKRQEEDCTFSIMENNGQPCINDDEEMKEVDLPEIEADSGCPQPVPPENGFIRNEKQLYLVGEDVEISCLTGFETVGYQYFRCLPDGTWRQGDVECQRTECIKPVVQEVLTITPFQRLYRIGESIELTCPKGFVVAGPSRYTCQGNSWTPPISNSLTCEKDTLTKLKGHCQLGQKQSGSECICMSPEEDCSHHSEDLCVFDTDSNDYFTSPACKFLAEKCLNNQQLHFLHIGSCQDGRQLEWGLERTRLSSNSTKKESCGYDTCYDWEKCSASTSKCVCLLPPQCFKGGNQLYCVKMGSSTSEKTLNICEVGTIRCANRKMEILHPGKCLAI

分子量

Approximately 105-120 kDa due to the glycosylation.

糖基化修饰
Yes
纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

C6/Complement component C6 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
C6/Complement component C6 Protein, Human (HEK293, His)
目录号:
HY-P74373
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