Capsid 蛋白, Hepatitis B Virus (His)

Capsid proteins play a key role in the assembly of icosahedral capsids, forming T=4 and T=3 particles. Transported along microtubules to the nucleus, its phosphorylation exposes a nuclear localization signal that promotes binding to nuclear pore complexes via α and β import. Capsid protein, Hepatitis B Virus (His) is the recombinant Virus-derived Capsid protein, expressed by E. coli , with C-His labeled tag. The total length of Capsid protein, Hepatitis B Virus (His) is 149 a.a., with molecular weight of ~17.67 kDa.

衣壳蛋白在二十面体衣壳的组装中起着至关重要的作用，形成由 240 个衣壳蛋白拷贝组成的具有 T=4 二十面体对称性的大颗粒，而一小部分则组装成具有 180 个衣壳蛋白的较小 T=3 颗粒。这些衣壳沿着微管运输到细胞核，其中衣壳的磷酸化诱导其C末端部分的核定位信号的暴露，从而促进通过导入蛋白（核传递蛋白-）α和β与核孔复合物的结合。完整的衣壳通过核孔进入核篮，可能与 NUP153 结合。只有含有病毒基因组的成熟衣壳才能将病毒DNA和衣壳蛋白释放到核质中，而未成熟的衣壳仍然卡在篮子里。衣壳封装前基因组RNA和P蛋白，RNA在细胞质中逆转录成DNA。逆转录后的衣壳可以定向到细胞核进行额外的转录，或者通过内质网出芽产生新的病毒颗粒。衣壳蛋白同二聚化，然后多聚化，并与网状到高尔基体区室中病毒 L 糖蛋白的胞质暴露区域相互作用。此外，它与人 FLNB 相互作用，并且以其磷酸化形式与宿主输入蛋白 α 相互作用，这取决于受基因组成熟和/或衣壳蛋白磷酸化影响的 NLS 暴露。衣壳蛋白还在病毒组装和基因组释放的复杂过程中与宿主 NUP153 相互作用。

Virus

E. coli

C-His

P03146-1 (M1-V149)

944568  [NCBI]

Approximately 17.67 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 150 mM NaCl, 50 mM Tris, 0.5 mM EDTA, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.