CDKN1A 蛋白, Human (GST)

The CDKN1A protein critically controls cell cycle progression and mediates DNA damage-induced G2 arrest through p53/TP53-dependent and CREB3L1-mediated p53-independent pathways. It inhibits cyclin-dependent kinase activity, blocking cell cycle progression by preventing phosphorylation of key substrates. CDKN1A Protein, Human (GST) is the recombinant human-derived CDKN1A protein, expressed by E. coli , with N-GST labeled tag. The total length of CDKN1A Protein, Human (GST) is 163 a.a., with molecular weight of 45 kDa.

CDKN1A 蛋白在控制细胞周期进程和协调 DNA 损伤诱导的 G2 停滞中发挥着至关重要的作用。它积极参与 p53/TP53 介导的 DNA 损伤介导的细胞增殖抑制，并且还参与星形胶质细胞和成骨细胞中由 CREB3L1 介导的 p53 独立 DNA 损伤诱导的 G2 停滞。从功能上讲，CDKN1A 结合并抑制细胞周期蛋白依赖性激酶活性，防止关键细胞周期蛋白依赖性激酶底物的磷酸化，从而阻断细胞周期进程。它有助于细胞周期蛋白 D-CDK4 复合物的核定位和组装，促进其对 RB1 的激酶活性。在较高的化学计量比下，CDKN1A 抑制细胞周期蛋白 D-CDK4 复合物的激酶活性，并与 POLD3 竞争 PCNA 结合，从而抑制 DNA 聚合酶 delta 的 DNA 合成。CDKN1A 复杂的调控网络包括与 HDAC1、MKRN1、PSMA3、CDK4、CDK2、PIM1、STK11、NUAK1 和 TRIM39 亚型的相互作用，反映了其在细胞周期调控和 DNA 损伤反应中的不同作用。

Human

E. coli

N-GST

P38936 (S2-P164)

1026  [NCBI]

SEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP

53 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.