CDKN2A 蛋白, Human

CDKN2A Protein, a potent negative regulator of cell proliferation, forms strong interactions with CDK4 and CDK6, hindering their association with cyclins D and retinoblastoma protein phosphorylation. Acting in a heterodimeric manner, predominantly with CDK6, CDKN2A inhibits cyclin D-CDK4 kinase activity and interacts with ISCO2, contributing to its cell cycle regulatory role. CDKN2A Protein, Human is the recombinant human-derived CDKN2A protein, expressed by E. coli , with tag free.

CDKN2A 蛋白通过与 CDK4 和 CDK6 形成强有力的相互作用，充当正常细胞增殖的有效负调节因子，从而阻止它们与细胞周期蛋白 D 的结合并阻碍视网膜母细胞瘤蛋白的磷酸化。它以异二聚体方式与 CDK4 或 CDK6 一起发挥作用，大多数 p16 复合物主要以 CDK6 为特征。与 CDK4 的相互作用以“T-172”磷酸化和非磷酸化形式发生，有效抑制细胞周期蛋白 D-CDK4 的激酶活性。此外，CDKN2A 蛋白与 ISCO2 结合，有助于其在细胞周期进程中的调节作用。

Human

E. coli

Tag Free

P42771 (E2-D156)

1029  [NCBI]

Partial

EPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD

Approximately 19.12 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.