1. 重组蛋白
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Contactin-1
  6. Contactin-1/CNTN1 蛋白, Mouse (HEK293, His)

Contactin-1/CNTN1 蛋白, Mouse (HEK293, His)

目录号: HY-P74224
产品使用指南

Contactin-1/CNTN1 蛋白在神经系统发育过程中介导表面相互作用。它在周围神经中形成节旁轴突-神经胶质连接,并通过 CNTNAP1 促进轴突-神经胶质信号传导。它作为 NOTCH1 的配体,促进 NOTCH1 激活。Contactin-1/CNTN1 还与 TNR 相互作用,抑制神经突生长。它与 CNTNAP1 和 PTPRZ1 结合,并与 NRCAM、PTPRB 和 TASOR 形成复合物。Contactin-1/CNTN1 蛋白, Mouse (HEK293, His) 是重组的 Contactin-1/CNTN1 蛋白,由 HEK293 表达,带有 C-His 标签。Contactin-1/CNTN1 蛋白, Mouse (HEK293, His) 全长 1000 个氨基酸,分子量为 110-120 kDa。

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描述

Contactin-1/CNTN1 protein mediates surface interactions during nervous system development. It forms paranodal axo-glial junctions in peripheral nerves and facilitates axon-glia signaling through CNTNAP1. It acts as a ligand for NOTCH1, promoting NOTCH1 activation. Contactin-1/CNTN1 also interacts with TNR, inhibiting neurite outgrowth. It binds to CNTNAP1 and PTPRZ1, and forms a complex with NRCAM, PTPRB, and TASOR. Contactin-1/CNTN1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Contactin-1/CNTN1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Contactin-1/CNTN1 Protein, Mouse (HEK293, His) is 1000 a.a., with molecular weight of 110-120 kDa.

研究背景

Contactin-1/CNTN1 蛋白在神经系统发育过程中介导细胞表面相互作用中发挥着至关重要的作用。它参与有髓周围神经中节旁轴-神经胶质连接的形成,并通过与 CNTNAP1 的关联促进轴突和髓鞘神经胶质细胞之间的信号传导。该蛋白还通过充当 NOTCH1 的配体参与少突胶质细胞的生成。Contactin-1/CNTN1 和 NOTCH1 之间的相互作用通过 Notch 胞内结构域 (NICD) 的释放以及随后易位至细胞核来促进 NOTCH1 的激活。此外,Contactin-1/CNTN1 与 TNR 相互作用,导致神经元排斥并抑制神经突生长。它以单体形式存在,并以顺式形式与 CNTNAP1 相互作用,同时与 PTPRZ1 的碳酸酐酶样结构域结合。此外,Contactin-1/CNTN1 在与 NRCAM 和 PTPRB 的复合物中被检测到,并与 TASOR 相互作用。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P12960 (M1-L1000)

基因 ID

12805  [NCBI]

蛋白结构
N-term
CNTN1 (M1-L1000)
Accession # P12960
His
C-term
同用名
CNTN1; Contactin1; F11; F3; Neural cell surface protein F3
分子量

110-120 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Contactin-1/CNTN1 Protein, Mouse (HEK293, His)
目录号:
HY-P74224
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