Cas9 蛋白, S. pyogenes (His)

The Cas9 protein is part of an immune system that defends against genetic elements. It processes RNA and aids in cleaving DNA targets. Protein and guide RNAs are necessary for its function. The protein recognizes specific sequences to distinguish self from nonself and provides immunity against matching genetic elements. Cas9 Protein, S. pyogenes (His) is the recombinant Cas9 Protein protein, expressed by E. coli , with His tagged.

该过程涉及 crRNA 的转录和加工，其中，对于 II 型 基因编辑系统，前 crRNA 的正确加工需要反式编码的小 RNA (tracrRNA)、内源性核糖核酸酶 3 (rnc) 和关键的 Cas9蛋白质。tracrRNA 引导核糖核酸酶 3 处理 pre-crRNA，Cas9 稳定 pre-crRNA:tracrRNA 相互作用。在后续步骤中，Cas9/crRNA/tracrRNA 复合物充当核酸内切酶，用互补的间隔序列切割靶 DNA。如果没有两个特定引导 RNA (gRNA) 的指导，Cas9 就会失活，这强调了这两种 gRNA 对于 DNA 结合和核酸酶活性的必要性。Cas9 对原型间隔子相邻基序 (PAM) 的识别对于区分自身与非自身至关重要，从而确保准确的目标识别。这个复杂的系统通过一种称为基因编辑干扰的机制赋予对入侵遗传元件的免疫力。

The cleavage activity in vitro was not less than 85%.

Others

E. coli

His

Q99ZW2 (D2-D1368)

/

Partial

Approximately 163 kDa

Greater than 95% as determined by Tris-Bis PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 30 mM Tris-HCl, 0.3 M NaCl, 50% glycerol, 0.1 mM EDTA, pH 7.4.

<0.01 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.