Cyclin-H/CCNH 蛋白, Human (His)

Cyclin-H/CCNH plays a key role as a modulator of CDK7, the catalytic subunit of the CDK-activated kinase (CAK) complex. This enzyme complex activates cyclin-related kinases such as CDK1, CDK2, CDK4, and CDK6 through threonine phosphorylation. Cyclin-H/CCNH Protein, Human (His) is the recombinant human-derived Cyclin-H/CCNH protein, expressed by E. coli , with N-6*His labeled tag.

Cyclin-H/CCNH 蛋白作为 CDK7 的关键调节因子发挥作用，CDK7 是 CDK 激活激酶 (CAK) 酶复合物的催化亚基。反过来，CAK 通过苏氨酸磷酸化在激活细胞周期蛋白相关激酶（例如 CDK1、CDK2、CDK4 和 CDK6）中发挥着至关重要的作用。与核心 TFIIH 基础转录因子结合，CAK 通过其大亚基 (POLR2A) 的重复 C 端结构域 (CTD) 的丝氨酸磷酸化促进 RNA 聚合酶 II 激活，从而使聚合酶逃离启动子并启动转录物延伸。Cyclin-H/CCNH 积极参与细胞周期控制和 RNA 聚合酶 II 的 RNA 转录，其表达和活性在整个细胞周期中保持恒定。它主要与CDK7和MAT1结合形成CAK复合物，CAK复合物又可以进一步与核心-TFIIH结合构成TFIIH基础转录因子。这些调节相互作用凸显了 Cyclin-H/CCNH 在协调关键细胞过程中的核心作用，这些过程对于正确的细胞周期进程和转录控制至关重要。

Human

E. coli

N-6*His

P51946 (M1-L323)

902  [NCBI]

Full Length

MYHNSSQKRHWTFSSEEQLARLRADANRKFRCKAVANGKVLPNDPVFLEPHEEMTLCKYYEKRLLEFCSVFKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSPQFVGNLRESPLGQEKALEQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDLKTRYPILENPEILRKTADDFLNRIALTDAYLLYTPSQIALTAILSSASRAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL

34-39 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 2 mM DTT, 30% glycerol, pH 8.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.