DC-SIGN/CD209 蛋白, Rhesus Macaque (HEK293, His)

DC-SIGN/CD209 Protein, is a C-type lectin receptor present on the surface of both macrophages and dendritic cells (DCs), serves as a pivotal pathogen-recognition receptor, playing a crucial role in initiating the primary immune response. It mediates endocytosis and subsequent degradation of pathogens within lysosomal compartments. On DCs, it acts as a high-affinity receptor for ICAM2 and ICAM3, binding to mannose-like carbohydrates. DC-SIGN/CD209 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived DC-SIGN/CD209 protein, expressed by HEK293 , with N-His labeled tag.

DC-SIGN/CD209 蛋白是一种存在于巨噬细胞和树突状细胞 (DC) 表面的C型凝集素受体，是关键的病原体识别受体，在启动初级免疫反应中发挥着至关重要的作用。该受体参与病原体的内吞作用，导致其随后在溶酶体室内降解。在此过程之后，DC-SIGN 返回细胞膜表面，通过 MHC II 类蛋白将病原体衍生的抗原呈递给静息 T 细胞，从而触发适应性免疫反应。在 DC 上，它充当 ICAM2 和 ICAM3 的高亲和力受体，与甘露糖样碳水化合物结合。值得注意的是，DC-SIGN 可能充当 DC 滚动受体，通过与内皮 ICAM2 相互作用，促进 DC 前体从血流跨内皮迁移到组织。此外，它似乎通过与 DC 和 T 细胞之间形成的免疫突触内 T 细胞上的 ICAM3 结合来调节 DC 诱导的 T 细胞增殖^[1][2]。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rhesus Macaque DC-SIGN at 2 μg/mL can bind gp120. The ED₅₀ for this effect is 1.092 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rhesus Macaque DC-SIGN at 2 μg/mL canbind gp120. The ED₅₀ for this effect is 1.092 μg/mL

Rhesus Macaque

HEK293

N-6*His

AAK74185.1 (K62-E381)

574211  [NCBI]

KVPSSLSQGQSKQDAIYQNLTQLKVAVSELSEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYEELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKQQEIYQELSRLKAAVGDLPEKSKQQEIYQKLTQLKAAVDGLPDRSKQQEIYQELIQLKAAVERLCRPCPWEWTFFQGNCYFMSNSQRNWHNSITACQEVGAQLVVIKSAEEQNFLQLQSSRSNRFTWMGLSDLNHEGTWQWVDGSPLLPSFKQYWNKGEPNNIGEEDCAEFSGNGWNDDKCNLAKFWICKKSAASCSGDEERLLSPAPTTPNPPPE

Approximately 44 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.