DEFA1 蛋白, Human (GST)

The DEFA1 protein is a multifunctional effector in innate immunity that exhibits antibiotic-like properties against bacteria, fungi, and viruses. It promotes immune defense by activating antigen-presenting cells (APCs) and inhibiting bacterial cell wall synthesis by interacting with lipid II. DEFA1 Protein, Human (GST) is the recombinant human-derived DEFA1 protein, expressed by E. coli , with N-GST labeled tag. The total length of DEFA1 Protein, Human (GST) is 94 a.a., with molecular weight of ~34 kDa.

DEFA1 蛋白是先天免疫系统中的多功能效应子，具有类似抗生素的特性，可对抗各种感染原，包括细菌、真菌和病毒。此外，DEFA1 通过促进抗原呈递细胞 (APC) 的激活和成熟来促进免疫防御。通过与细胞壁合成的重要前体脂质 II 相互作用，DEFA1 抑制细菌细胞壁的合成。它通过抑制病毒在顶点区域的分解来进一步阻止腺病毒感染，从而阻碍内部衣壳蛋白 pVI 的释放，这对于细胞进入过程中内体膜的渗透至关重要。该蛋白与腺病毒衣壳的相互作用将病毒颗粒重定向至 TLR4，促进 NLRP3 介导的炎症反应和白细胞介素 1 β (IL-1β) 释放。DEFA1 通过触发 NFKBIA 降解和 IRF1 核转位，诱导浆细胞样树突状细胞 (pDC) 中促炎细胞因子的产生，包括 I 型干扰素，这两者对于 pDC 激活都至关重要。在结构上，DEFA1 以四聚体和二聚体的形式存在，说明了其动态组织，并且它与 RETN 相互作用。

Human

E. coli

N-GST

P59665 (D1-C94)

1667  [NCBI]

DIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC

Approximately 34 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.